Proteintech

PAK4 Polyclonal Antibody

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Antibody Testing Data (12)

PAK4 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

PAK4 Antibody (14685-1-AP) in ICC/IF

Immunofluorescent analysis of Hela cells, using PAK4 antibody 14685-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Product Image Gallery

PAK4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human prostate cancer using 14685-1-AP (PAK4 antibody) at dilution of 1:100 (under 10x lens).

PAK4 Antibody in Western Blot (WB) — Rat skin tissue were subjected to SDS PAGE followed by western blot with 14685-1-AP (PAK4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

PAK4 Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.2ug PAK4 antibody (14685-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

PAK4 Antibody in Immunoprecipitation (IP) — IP result of anti-PAK4 (IP:14685-1-AP, 4ug; Detection:14685-1-AP 1:800) with mouse liver tissue lysate 4000ug.

Product Details

14685-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 5 publications 5 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PAK4 Fusion Protein Ag5981 (1-350 aa encoded by BC025282)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.51 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MFGKRKKRV EISAPSNFEH RVHTGFDQHE QKFTGLPRQW QSLIEESARR PKPLVDPACI TSIQPGAPKT IVRGSKGAKD GALTLLLDEF ENMSVTRSNS LRRDSPPPPA RARQENGMPE EPATTARGGP GKAGSRGRFA GHSEAGGGSG DRRRAGPEKR PKSSREGSGG PQESSRDKRP LSGPDVGTPQ PAGLASGAKL AAGRPFNTYP RADTDHPSRG AQGEPHDVAP NGPSAGGLAI PQSSSSSSRP PTRARGAPSP GVLGPHASEP QLAPPACTPA APAVPGPPGP RSPQREPQRV SHEQFRAALQ LVVDPGDPRS YLDNFIKIGE GSTGIVCIAT VRSSGKLVAV K (1-350 aa encoded by BC025282)

Target Information

PAK4 is a serine/ threonine p21 activating kinase implicated in the reorganization of actin cytoskeleton and in the formation of filopodia. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. The second group includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK4 was initially identified as a novel effector of Cdc42Hs. Co-expression of PAK4 and Cdc42Hs resulted in induction of filopodia and actin polymerization, showing that it is involved in cytoskeletal reorganization. Other experiments have shown PAK4 to be essential for embryonic viability and proper neuronal development. PAK4 has also been implicated in anchorage-independent growth of tumor cells and is required for activation of several cancer prosurvival pathways.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Expression Patterns of PAK4 and PHF8 Are Associated with the Survival of Gallbladder Carcinoma Patients.

Diagnostics (Basel, Switzerland)

Ahn AR,Karamikheirabad M,Park MS,Zhang J,Kim HS,Jeong JS,Kim KM,Park HS,Jang KY

Fri Mar 17 00:00:00 EDT 2023

Pan-cancer analysis identifies the immunological and prognostic role of PAK4.

Life sciences

Tang L,Gao Y,Li T

Sun Jan 01 00:00:00 EST 2023

Pan-cancer analysis identifies the immunological and prognostic role of PAK4.

Life sciences

Tang L,Gao Y,Li T

Sun Jan 01 00:00:00 EST 2023

RhoGDIα regulates spermatogenesis through Rac1/cofilin/F-actin signaling.

Communications biology

Zhu H,Wen Z,Zhang A,Liu D,Wang H,Cheng Y,Yang X,Xiao Y,Li J,Sun D,Wu B,Gao J

Thu Feb 23 00:00:00 EST 2023

Bacterial infection promotes tumorigenesis of colorectal cancer via regulating CDC42 acetylation.

PLoS pathogens

Wang DN,Ni JJ,Li JH,Gao YQ,Ni FJ,Zhang ZZ,Fang JY,Lu J,Yao YF

Wed Feb 01 00:00:00 EST 2023

The early molecular events leading to COFILIN phosphorylation during mouse sperm capacitation are essential for acrosomal exocytosis.

The Journal of biological chemistry

Schiavi-Ehrenhaus LJ,Romarowski A,Jabloñski M,Krapf D,Luque GM,Buffone MG

Wed Jun 01 00:00:00 EDT 2022

The early molecular events leading to COFILIN phosphorylation during mouse sperm capacitation are essential for acrosomal exocytosis.

The Journal of biological chemistry

Schiavi-Ehrenhaus LJ,Romarowski A,Jabloñski M,Krapf D,Luque GM,Buffone MG

Wed Jun 01 00:00:00 EDT 2022

Proximity proteomics identifies PAK4 as a component of Afadin-Nectin junctions.

Nature communications

Baskaran Y,Tay FP,Ng EYW,Swa CLF,Wee S,Gunaratne J,Manser E

Tue Sep 07 00:00:00 EDT 2021

Proximity proteomics identifies PAK4 as a component of Afadin-Nectin junctions.

Nature communications

Baskaran Y,Tay FP,Ng EYW,Swa CLF,Wee S,Gunaratne J,Manser E

Tue Sep 07 00:00:00 EDT 2021

Targeting PAK4 Inhibits Ras-Mediated Signaling and Multiple Oncogenic Pathways in High-Risk Rhabdomyosarcoma.

Cancer research

Dasgupta A,Sierra L,Tsang SV,Kurenbekova L,Patel T,Rajapakse K,Shuck RL,Rainusso N,Landesman Y,Unger T,Coarfa C,Yustein JT

Fri Jan 01 00:00:00 EST 2021

Targeting PAK4 Inhibits Ras-Mediated Signaling and Multiple Oncogenic Pathways in High-Risk Rhabdomyosarcoma.

Cancer research

Dasgupta A,Sierra L,Tsang SV,Kurenbekova L,Patel T,Rajapakse K,Shuck RL,Rainusso N,Landesman Y,Unger T,Coarfa C,Yustein JT

Fri Jan 01 00:00:00 EST 2021

ZEB1-Mediated Transcriptional Upregulation of circWWC3 Promotes Breast Cancer Progression through Activating Ras Signaling Pathway.

Molecular therapy. Nucleic acids

Meng L,Liu S,Liu F,Sang M,Ju Y,Fan X,Gu L,Li Z,Geng C,Sang M

Wed Aug 19 00:00:00 EDT 2020

ZEB1-Mediated Transcriptional Upregulation of circWWC3 Promotes Breast Cancer Progression through Activating Ras Signaling Pathway.

Molecular therapy. Nucleic acids

Meng L,Liu S,Liu F,Sang M,Ju Y,Fan X,Gu L,Li Z,Geng C,Sang M

Wed Aug 19 00:00:00 EDT 2020

PAK4 suppresses TNF-induced release of endothelial microparticles in HUVECs cells.

Aging

Zhang S,Yin Y,Li C,Zhao Y,Wang Q,Zhang X

Sun Jul 12 00:00:00 EDT 2020

PAK4 inhibition improves PD-1 blockade immunotherapy.

Nature cancer

Abril-Rodriguez G,Torrejon DY,Liu W,Zaretsky JM,Nowicki TS,Tsoi J,Puig-Saus C,Baselga-Carretero I,Medina E,Quist MJ,Garcia AJ,Senapedis W,Baloglu E,Kalbasi A,Cheung-Lau G,Berent-Maoz B,Comin-Anduix B,Hu-Lieskovan S,Wang CY,Grasso CS,Ribas A

Thu Apr 14 00:00:00 EDT 2022

PAK4 inhibition improves PD-1 blockade immunotherapy.

Nature cancer

Thu Apr 14 00:00:00 EDT 2022

PAK4 inhibition improves PD-1 blockade immunotherapy.

Nature cancer

Thu Apr 14 00:00:00 EDT 2022

PAK4 inhibition improves PD-1 blockade immunotherapy.

Nature cancer

Thu Apr 14 00:00:00 EDT 2022

Nonconserved miR-608 suppresses prostate cancer progression through RAC2/PAK4/LIMK1 and BCL2L1/caspase-3 pathways by targeting the 3'-UTRs of RAC2/BCL2L1 and the coding region of PAK4.

Cancer medicine

Zhang X,Fang J,Chen S,Wang W,Meng S,Liu B

Sun Sep 01 00:00:00 EDT 2019

Nonconserved miR-608 suppresses prostate cancer progression through RAC2/PAK4/LIMK1 and BCL2L1/caspase-3 pathways by targeting the 3'-UTRs of RAC2/BCL2L1 and the coding region of PAK4.

Cancer medicine

Zhang X,Fang J,Chen S,Wang W,Meng S,Liu B

Sun Sep 01 00:00:00 EDT 2019

Normal mammary gland development after MMTV-Cre mediated conditional PAK4 gene depletion.

Scientific reports

Rabieifar P,Zhuang T,Costa TDF,Zhao M,Strömblad S

Tue Oct 08 00:00:00 EDT 2019

Normal mammary gland development after MMTV-Cre mediated conditional PAK4 gene depletion.

Scientific reports

Rabieifar P,Zhuang T,Costa TDF,Zhao M,Strömblad S

Tue Oct 08 00:00:00 EDT 2019

Trio Haploinsufficiency Causes Neurodevelopmental Disease-Associated Deficits.

Cell reports

Katrancha SM,Shaw JE,Zhao AY,Myers SA,Cocco AR,Jeng AT,Zhu M,Pittenger C,Greer CA,Carr SA,Xiao X,Koleske AJ

Tue Mar 05 00:00:00 EST 2019

CCM2 and PAK4 act downstream of atrial natriuretic peptide signaling to promote cell spreading.

The Biochemical journal

Miura K,Nojiri T,Akitake Y,Ando K,Fukuhara S,Zenitani M,Kimura T,Hino J,Miyazato M,Hosoda H,Kangawa K

Fri May 19 00:00:00 EDT 2017

CCM2 and PAK4 act downstream of atrial natriuretic peptide signaling to promote cell spreading.

The Biochemical journal

Miura K,Nojiri T,Akitake Y,Ando K,Fukuhara S,Zenitani M,Kimura T,Hino J,Miyazato M,Hosoda H,Kangawa K

Fri May 19 00:00:00 EDT 2017

MiR-145 inhibits human colorectal cancer cell migration and invasion via PAK4-dependent pathway.

Cancer medicine

Sheng N,Tan G,You W,Chen H,Gong J,Chen D,Zhang H,Wang Z

Thu Jun 01 00:00:00 EDT 2017

MiR-145 inhibits human colorectal cancer cell migration and invasion via PAK4-dependent pathway.

Cancer medicine

Sheng N,Tan G,You W,Chen H,Gong J,Chen D,Zhang H,Wang Z

Thu Jun 01 00:00:00 EDT 2017

The Cdc42 Effector Kinase PAK4 Localizes to Cell-Cell Junctions and Contributes to Establishing Cell Polarity.

PloS one

Selamat W,Tay PL,Baskaran Y,Manser E

Fri May 13 00:00:00 EDT 2016

The Cdc42 Effector Kinase PAK4 Localizes to Cell-Cell Junctions and Contributes to Establishing Cell Polarity.

PloS one

Selamat W,Tay PL,Baskaran Y,Manser E

Fri May 13 00:00:00 EDT 2016

The Cdc42 Effector Kinase PAK4 Localizes to Cell-Cell Junctions and Contributes to Establishing Cell Polarity.

PloS one

Selamat W,Tay PL,Baskaran Y,Manser E

Fri May 13 00:00:00 EDT 2016

The Cdc42 Effector Kinase PAK4 Localizes to Cell-Cell Junctions and Contributes to Establishing Cell Polarity.

PloS one

Selamat W,Tay PL,Baskaran Y,Manser E

Fri May 13 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: p21 (CDKN1A)-activated kinase 4; p21 protein (Cdc42/Rac)-activated kinase 4; p21(CDKN1A)-activated kinase 4; p21-activated kinase 4; PAK-4; protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs; Serine/threonine-protein kinase PAK 4

Gene Aliases: 5730488L07Rik; AW555722; KIAA1142; mKIAA1142; PAK4

UniProt ID: (Human) O96013, (Mouse) Q8BTW9

Entrez Gene ID: (Human) 10298, (Mouse) 70584, (Rat) 292756

Molecular Function: non-receptor serine/threonine protein kinase