|Tested species reactivity||Human, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: C E(324) Q L K Q E N K T L L K V V G Q L T R(342)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 2 publications below|
PA1-755 detects prostate apoptosis response protein (Par-4) from human and rat tissues.
PA1-755 has been sucessfully used in Western blot procedures. By Western blot, this antibody detects an ~40 kDa protein representing Par-4 in AT-3 cell extract. In Western blots of rat tissue extracts, including testes, spleen, ovary, lung, and skeletal muscle, ~40 kDa and ~38 kDa proteins representing Par-4 are detected. In Western blots of T47D cell extracts, an ~40 kDa protein representing Par-4 and a smaller ~20 kDa unknown protein is detected.
PA1-755 immunizing peptide corresponds to amino acids residues 324-342 from human Par-4. PA1-755 immunizing peptide (Cat. # PEP-060) is available for use in neutralization and control experiments.
Prostate apoptosis response protein (Par-4) is an ~38 kDa protein containing a leucine zipper at its carboxy terminus. The leucine zipper is required for Par-4 function during apoptosis and has also been shown to directly interact with Wilm and quote;s Tumor Suppressor, WT-1. Par-4 is up-regulated in apoptotic prostate cells and has also been observed at ~18 times higher concentration in human brain tissue from Alzheimer's patients when compared to unaffected brain tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Par-4 is a mediator of neuronal degeneration associated with the pathogenesis of Alzheimer disease.
PA1-755 was used in immunohistochemistry, western blot and immunocytochemistry to investigate the role of Par-4 in neuronal degeneration with respect to Alzheimer disease
|Guo Q,Fu W,Xie J,Luo H,Sells SF,Geddes JW,Bondada V,Rangnekar VM,Mattson MP||Nature medicine (4:957)||1998|
|Not Applicable||Not Cited||
A novel repressor, par-4, modulates transcription and growth suppression functions of the Wilms' tumor suppressor WT1.
PA1-755 was used in immunoprecipitation and western blot to investigate the effect of par-4 on WT1 transcription
|Johnstone RW,See RH,Sells SF,Wang J,Muthukkumar S,Englert C,Haber DA,Licht JD,Sugrue SP,Roberts T,Rangnekar VM,Shi Y||Molecular and cellular biology (16:6945)||1996|