This antibody was generated against the N-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.
Immunogen sequence: MAESSDKLY RVEYAKSGRA SCKKCSESIP KDSLRMAIMV QSPMFDGKVP HWYHFSCFWK VGHSIRHPDV EVDGFSELRW DDQQKVKKTA EAGGVTGKGQ DGIGSKAEKT LGDFAAEYAK SNRSTCKGCM EKIEKGQVRL SKKMVDPEKP QLGMIDRWYH PGCFVKNREE LGFRPEYSAS QLKGFSLLAT EDKEALKKQL PGVKSEGKRK GDEVDGVDEV AKKKSKKEKD KDSKLEKALK AQNDLIWNIK DELKKVCSTN DLKELLIFNK QQVPSGESAI LDRVADGMVF GALLPCEECS GQLVFKSDAY YCTGDVTAWT KCMVKTQT (1-327 aa encoded by BC037545)
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); ADPRT 1; ARTD1; DNA ADP-ribosyltransferase PARP1; msPARP; NAD(+) ADP-ribosyltransferase 1; NAD(+) pADPRT-1; NT-PARP-1; PARP-1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; Poly [ADP-ribose] polymerase 1; Poly [ADP-ribose] polymerase 1, 24-kDa form; Poly [ADP-ribose] polymerase 1, 28-kDa form; Poly [ADP-ribose] polymerase 1, 89-kDa form; Poly [ADP-ribose] polymerase 1, processed C-terminus; Poly [ADP-ribose] polymerase 1, processed N-terminus; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; poly[ADP-ribose] synthetase 1; Protein poly-ADP-ribosyltransferase PARP1
Gene Aliases: 5830444G22Rik; Adprp; ADPRT; ADPRT 1; ADPRT1; AI893648; ARTD1; C80510; pADPRT-1; PARP; PARP-1; PARP1; PPOL; sPARP-1
UniProt ID: (Human) P09874
Molecular Function: DNA metabolism protein