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Invitrogen
PARP12 Polyclonal Antibody
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FIGURE: 1 / 1
PARP12 Antibody (PA5-89749) in WB
Western blot analysis of PARP12 in extracts of various cell lines. Samples were incubated with PARP12 Polyclonal antibody (Product # PA5-89749) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-89749
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein (or fragment)
Conjugate
Form
Liquid
Concentration
1.84 mg/mL
Amount
184 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: YVSPQDVTTM QTCNTKFPGP KSIPDYWDSS ALPDPGFQKI TLSSSSEEYQ KVWNLFNRTL PFYFVQKIER VQNLALWEVY QWQKGQMQKQ NGGKAVDERQ LFHGTSAIFV DAICQQNFDW RVCGVHGTSY GKGSYFARDA AYSHHYSKSD TQTHTMFLAR VLVGEFVRGN ASFVRPPAKE GWSNAFYDSC VNSVSDPSIF VIFEKHQVYP EYVIQYTTSS KPSVTPSILL ALGSLFSSRQ; Positive Samples: HT-29, HeLa, Mouse brain, Mouse liver, Mouse pancreas, Rat brain; Cellular Location: Nucleus
Target Information
Poly(ADP-ribosyl)ation is an immediate DNA-damage-dependent post-translational modification of histones and other nuclear proteins that contributes to the survival of injured proliferating cells. Poly(ADP-ribose) polymerases (PARPs) now constitute a large family of 18 proteins, encoded by different genes and displaying a conserved catalytic domain in which PARP-1 (113 kDa), the founding member, and PARP-2 (62 kDa) are so far the sole enzymes whose catalytic activity has been shown to be immediately stimulated by DNA strand breaks. A large repertoire of sequences encoding novel PARPs now extends considerably the field of poly(ADP-ribosyl)ation reactions to various aspects of the cell biology including cell proliferation and cell death. Some of these new members interact with each other, share common partners and common subcellular localizations suggesting possible fine tuning in the regulation of this post-translational modification of proteins.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ADP-ribosyltransferase diphtheria toxin-like 12; ARTD12; PARP-12; Poly [ADP-ribose] polymerase 12; poly ADP-ribose polymerase 12; Protein mono-ADP-ribosyltransferase PARP12; unnamed protein product; Zinc finger CCCH domain-containing protein 1; zinc finger CCCH type domain containing 1
Gene Aliases: 9930021O16; AA409132; AA536654; ARTD12; MST109; MSTP109; PARP-12; PARP12; ZC3H1; ZC3HDC1
UniProt ID: (Human) Q9H0J9, (Mouse) Q8BZ20
Entrez Gene ID: (Human) 64761, (Rat) 362343, (Mouse) 243771
RNA binding
NAD+ ADP-ribosyltransferase activity
zinc ion binding
transferase activity
transferase activity, transferring glycosyl groups
nucleotidyltransferase activity
metal ion binding
NAD+-protein-cysteine ADP-ribosyltransferase activity
NAD+-protein-aspartate ADP-ribosyltransferase activity
protein ADP-ribosylase activity
poly(A) RNA binding
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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