Proteintech

PASK Polyclonal Antibody

View all (19) PASK antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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PASK Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HeLa cells using 14396-1-AP (PASK antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PASK Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human prostate hyperplasia tissue slide using 14396-1-AP (PASK Antibody) at dilution of 1:50 (under 40x lens).

PASK Antibody in Western Blot (WB) — HEK-293T cells were subjected to SDS PAGE followed by western blot with 14396-1-AP (PASK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

PASK Antibody in Immunoprecipitation (IP) — IP result of anti-PASK (IP:14396-1-AP, 4 ug; Detection:14396-1-AP 1:300) with K-562 cells lysate 4000 ug.

Product Details

14396-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PASK Fusion Protein Ag5893 (999-1323 aa encoded by BC063585) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.26 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: YSTMSPLGS GAFGFVWTAV DKEKNKEVVV KFIKKEKVLE DCWIEDPKLG KVTLEIAILS RVEHANIIKV LDIFENQGFF QLVMEKHGSG LDLFAFIDRH PRLDEPLASY IFRQLVSAVG YLRLKDIIHR DIKDENIVIA EDFTIKLIDF GSAAYLERGK LFYTFCGTIE YCAPEVLMGN PYRGPELEMW SLGVTLYTLV FEENPFCELE ETVEAAIHPP YLVSKELMSL VSGLLQPVPE RRTTLEKLVT DPWVTQPVNL ADYTWEEVFR VNKPESGVLS AASLEMGNRS LSDVAQAQEL CGGPVPGEAP NGQGCLHPGD PRLLTS (999-1323 aa encoded by BC063585 )

Target Information

PASKIN (PAS-Kinase) is thought to regulate protein synthesis in cells. It is a conserved gene product found in yeast as well as mammals. PASKIN contains two domains (PAS A and PAS B) as well as a serine/threonine kinase domain related to AMP kinases. PASKIN activity leads to decreased carbohydrate storage, as well as increased protein synthesis. PASKIN-dependent phosphorylation inhibits the activity of mammalian glycogen synthase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.