Immunofluorescent analysis of PASKIN (green) showing staining in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a PASKIN monoclonal antibody (Product # MA1-700) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Non-human primate, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant, GST tagged, PAS fusion protein expressed in E. coli.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
MA1-700 contains 100 ug of Protein A/G purified, in vitro produced IgG2a (1 mg/ml) in PBS with 1 mg/ml BSA and 0.05% sodium azide.
MA1-700 detects PASK from human, non-human primate and rat samples.
MA1-700 has been successfully used in Western blot and CC/IF procedures.
The MA1-700 immunogen is recombinant, GST tagged, PAS fusion protein expressed in E. coli
This monoclonal antibody was expressed by hybridoma cells grown in vitro in ABR and quote;s dialysis-based bioreactor system. All media, reagents, and other variables that affect the system are continuously monitored and controlled, ensuring a superior degree of precision and repeatability compared to an in vivo ascites production. Additionally, the serum free medium is further protein purified for improved antibody performance.
PASKIN (PAS-Kinase) is thought to regulate protein synthesis in cells. It is a conserved gene product found in yeast as well as mammals. PASKIN contains two domains (PAS A and PAS B) as well as a serine/threonine kinase domain related to AMP kinases. PASKIN activity leads to decreased carbohydrate storage, as well as increased protein synthesis. PASKIN-dependent phosphorylation inhibits the activity of mammalian glycogen synthase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Male germ cell expression of the PAS domain kinase PASKIN and its novel target eukaryotic translation elongation factor eEF1A1.
MA1-700 was used in immunohistochemistry to study the PASKIN expression in male germ cell and its novel target eEF1A1
|Eckhardt K,Troger J,Reissmann J,Katschinski DM,Wagner KF,Stengel P,Paasch U,Hunziker P,Borter E,Barth S,Schlafli P,Spielmann P,Stiehl DP,Camenisch G,Wenger RH||Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (20:227)||2007|