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Invitrogen
PASK Monoclonal Antibody (mAB6)
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FIGURE: 1 / 3
PASK Antibody (MA1-700) in ICC/IF
Immunofluorescent analysis of PASKIN (green) showing staining in the cytoplasm of COS7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a PASKIN monoclonal antibody (Product # MA1-700) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjug... View More
Product Details
MA1-700
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
mAB6
Immunogen
Recombinant, GST tagged, PAS fusion protein expressed in E. coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
Product Specific Information
MA1-700 contains 100 µg of Protein A/G purified, in vitro produced IgG2a (1 mg/mL) in PBS with 1 mg/mL BSA and 0.05% sodium azide.
MA1-700 detects PASK from human, non-human primate and rat samples.
MA1-700 has been successfully used in Western blot and CC/IF procedures.
The MA1-700 immunogen is recombinant, GST tagged, PAS fusion protein expressed in E. coli
This monoclonal antibody was expressed by hybridoma cells grown in vitro in ABR's dialysis-based bioreactor system. All media, reagents, and other variables that affect the system are continuously monitored and controlled, ensuring a superior degree of precision and repeatability compared to an in vivo ascites production. Additionally, the serum free medium is further protein purified for improved antibody performance.
Target Information
PASK is an evolutionarily conserved protein present in yeast, flies, and mammals. PAS domains regulate the function of many intracellular signaling pathways in response to both extrinsic and intrinsic stimuli. It is a conserved gene product found in yeast as well as mammals. PASKIN contains two domains (PAS A and PAS B) as well as a serine/threonine kinase domain related to AMP kinases. PASKIN activity leads to decreased carbohydrate storage, as well as increased protein synthesis. PASKIN-dependent phosphorylation inhibits the activity of mammalian glycogen synthase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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