|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Western Blot (WB)||Assay dependent|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Human, Non-human primate, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant, GST tagged, PAS fusion protein expressed in E. coli.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-700 contains 100 ug of Protein A/G purified, in vitro produced IgG2a (1 mg/ml) in PBS with 1 mg/ml BSA and 0.05% sodium azide.
MA1-700 detects PASK from human, non-human primate and rat samples.
MA1-700 has been successfully used in Western blot and CC/IF procedures.
The MA1-700 immunogen is recombinant, GST tagged, PAS fusion protein expressed in E. coli
This monoclonal antibody was expressed by hybridoma cells grown in vitro in ABR and quote;s dialysis-based bioreactor system. All media, reagents, and other variables that affect the system are continuously monitored and controlled, ensuring a superior degree of precision and repeatability compared to an in vivo ascites production. Additionally, the serum free medium is further protein purified for improved antibody performance.
PASKIN (PAS-Kinase) is thought to regulate protein synthesis in cells. It is a conserved gene product found in yeast as well as mammals. PASKIN contains two domains (PAS A and PAS B) as well as a serine/threonine kinase domain related to AMP kinases. PASKIN activity leads to decreased carbohydrate storage, as well as increased protein synthesis. PASKIN-dependent phosphorylation inhibits the activity of mammalian glycogen synthase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: PAS domain-containing serine/threonine-protein kinase; PAS-Kinase; per-arnt-sim (PAS) domain kinase
Gene Aliases: KIAA0135; PASK; PASKIN; STK37
UniProt ID: (Human) Q96RG2
Entrez Gene ID: (Human) 23178
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