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|Tested species reactivity||Human, Mouse, Sheep, Rat, Xenopus, Zebrafish|
|Published species reactivity||Zebrafish, Mouse, Human, Chicken|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A GST-Pax-2 fusion protein derived from the C-terminal domain (aa188-385) of the murine Pax-2 protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Paraffin) (IHC (P))||See 7 publications below|
|Immunocytochemistry (ICC)||See 6 publications below|
|Immunohistochemistry (IHC)||See 16 publications below|
|Immunofluorescence (IF)||See 4 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 4 publications below|
|Western Blot (WB)||See 3 publications below|
The pax genes are a family of transcription factors that are active in specific tissues during early embryonic development. Pax family members possess a DNA-binding domain encoded by the paired box. Because paired domain containing genes encode transcription factors, they are capable of executing a genetic program. Several pax genes have also been associated with developmental mutations including: pax-3, which is associated with Waardenburg syndrome, pax-6, which is associated with Aniridia, and pax-2, which is associated with Wilms tumor. During embryogenesis, Pax-2 is expressed in the developing kidney. In particular, the pax-2 gene is expressed in condensing metanephric mesenchyme and in early epithelial structures derived from mesenchyme; however, pax-2 mRNA and protein levels are rapidly down regulated as the tubular epithelium matures. Although Pax-2 is down regulated during renal epithelium maturation, Pax-2 expression persists in the undifferentiated epithelium of Wilms' tumors. Persistent expression of Pax-2 in Wilms' tumors occurs frequently and correlates with the proliferation of poorly differentiated epithelial cells in these tumors. Interestingly, expression of the Wilms' tumor suppresser protein, WT1, coincides with down-regulation of the pax-2 gene; thus, suggesting that WT1 can directly repress pax-2 transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mesencephalic origin of the rostral Substantia nigra pars reticulata.
71-6000 was used in immunohistochemistry - paraffin section to determine the origin of the rostral Substantia nigra pars reticulata
|Madrigal MP,Moreno-Bravo JA,Martínez-López JE,Martínez S,Puelles E||Brain structure & function (221:1403)||2016|
Dissociation of embryonic kidney followed by re-aggregation as a method for chimeric analysis.
71-6000 was used in immunohistochemistry - paraffin section to describe three methods for re-constructing mouse fetal kidney tissue.
|Davies JA,Unbekandt M,Ineson J,Lusis M,Little MH||Methods in molecular biology (Clifton, N.J.) (886:135)||2012|
Fstl1 antagonizes BMP signaling and regulates ureter development.
71-6000 was used in immunohistochemistry - paraffin section to study the antagonization of BMP signaling by Fstl and the significance for normal development of the ureter.
|Xu J,Qi X,Gong J,Yu M,Zhang F,Sha H,Gao X||PloS one (7:null)||2012|
Calcium/NFAT signalling promotes early nephrogenesis.
71-6000 was used in immunohistochemistry - paraffin section to study the role of non-canonical Calcium/NFAT Wnt signaling in nephrogenesis.
|Burn SF,Webb A,Berry RL,Davies JA,Ferrer-Vaquer A,Hadjantonakis AK,Hastie ND,Hohenstein P||Developmental biology (352:288)||2011|
VHL mutations and dysregulation of pVHL- and PTEN-controlled pathways in multilocular cystic renal cell carcinoma.
71-6000 was used in immunohistochemistry - paraffin section to characterize multilocular cystic renal cell carcinoma samples.
|von Teichman A,Compérat E,Behnke S,Storz M,Moch H,Schraml P||Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (24:571)||2011|
Secretory cell outgrowth, PAX2 and serous carcinogenesis in the Fallopian tube.
71-6000 was used in immunohistochemistry - paraffin section to examine p53 and PAX2 in all secretory cell outgrowths.
|Chen EY,Mehra K,Mehrad M,Ning G,Miron A,Mutter GL,Monte N,Quade BJ,McKeon FD,Yassin Y,Xian W,Crum CP||The Journal of pathology (222:110)||2010|
Paired-Box genes are frequently expressed in cancer and often required for cancer cell survival.
71-6000 was used in immunohistochemistry - paraffin section and western blot to assess the pattern and requirement for PAX gene expression in a panel of common cancer cell lines.
|Muratovska A,Zhou C,He S,Goodyer P,Eccles MR||Oncogene (22:7989)||2003|
Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis.
71-6000 was used in immunocytochemistry to evaluate the kidney organoids from human iPS cells
|Takasato M,Er PX,Chiu HS,Maier B,Baillie GJ,Ferguson C,Parton RG,Wolvetang EJ,Roost MS,Chuva de Sousa Lopes SM,Little MH||Nature (526:564)||2015|
A transcription factor network specifying inhibitory versus excitatory neurons in the dorsal spinal cord.
71-6000 was used in immunocytochemistry to elucidate the roles of Ascl1 and Ptf1a in neuronal development
|Borromeo MD,Meredith DM,Castro DS,Chang JC,Tung KC,Guillemot F,Johnson JE||Development (Cambridge, England) (141:2803)||2014|
Directing human embryonic stem cell differentiation towards a renal lineage generates a self-organizing kidney.
71-6000 was used in immunocytochemistry to describe a protocol for pluripotent-stem-cell-based renal regeneration.
|Takasato M,Er PX,Becroft M,Vanslambrouck JM,Stanley EG,Elefanty AG,Little MH||Nature cell biology (16:118)||2014|
Primary cultures of glomerular parietal epithelial cells or podocytes with proven origin.
71-6000 was used in immunocytochemistry and western blot to examine the morphology of primary glomerular epithelial cells.
|Kabgani N,Grigoleit T,Schulte K,Sechi A,Sauer-Lehnen S,Tag C,Boor P,Kuppe C,Warsow G,Schordan S,Mostertz J,Chilukoti RK,Homuth G,Endlich N,Tacke F,Weiskirchen R,Fuellen G,Endlich K,Floege J,Smeets B,Moeller MJ||PloS one (7:null)||2012|
Wnt1 expression temporally allocates upper rhombic lip progenitors and defines their terminal cell fate in the cerebellum.
71-6000 was used in immunocytochemistry to study the Wnt expressing progenitors in the rhombomere.
|Hagan N,Zervas M||Molecular and cellular neurosciences (49:217)||2012|
|Mouse||Not Cited||Development of "Pinceaux" formations and dendritic translocation of climbing fibers during the acquisition of the balance between glutamatergic and gamma-aminobutyric acidergic inputs in developing Purkinje cells.||Sotelo C||The Journal of comparative neurology (506:240)||2008|
Sox9 is critical for suppression of neurogenesis but not initiation of gliogenesis in the cerebellum.
71-6000 was used in immunohistochemistry to investigate the role of Sox-9 in the maintenance and fate decision of the neural progenitor pool in the cerebellum.
|Vong KI,Leung CK,Behringer RR,Kwan KM||Molecular brain (8:null)||2015|
Expression of gastrin-releasing peptide by excitatory interneurons in the mouse superficial dorsal horn.
71-6000 was used in immunohistochemistry to compare the localization of gastrin-releasing peptide immunoreactivity to primary afferent or excitatory interneuron terminals
|Gutierrez-Mecinas M,Watanabe M,Todd AJ||Molecular pain (10:null)||2015|
Primary cilia function regulates the length of the embryonic trunk axis and urogenital field in mice.
71-6000 was used in immunohistochemistry to investigate mechanisms by which organs coordinate their size and shape
|Wainwright EN,Svingen T,Ng ET,Wicking C,Koopman P||Developmental biology (395:342)||2014|
Mdm2 is required for maintenance of the nephrogenic niche.
71-6000 was used in immunohistochemistry to investigate the role of Mdm2 in the nephrogenic niche.
|Hilliard SA,Yao X,El-Dahr SS||Developmental biology (387:1)||2014|
Parcellation of cerebellins 1, 2, and 4 among different subpopulations of dorsal horn neurons in mouse spinal cord.
71-6000 was used in immunohistochemistry to study the function of cerebellins in the cerebellum and striatum
|Cagle MC,Honig MG||The Journal of comparative neurology (522:479)||2014|
|Mouse||1:400||Otx2 cell-autonomously determines dorsal mesencephalon versus cerebellum fate independently of isthmic organizing activity.||Di Giovannantonio LG,Di Salvio M,Omodei D,Prakash N,Wurst W,Pierani A,Acampora D,Simeone A||Development (Cambridge, England) (141:377)||2014|
Chronic unilateral ureteral obstruction in the neonatal mouse delays maturation of both kidneys and leads to late formation of atubular glomeruli.
71-6000 was used in immunohistochemistry to examine the proximal nephron response to unilateral ureteral obstruction in the maturing kidney.
|Forbes MS,Thornhill BA,Galarreta CI,Minor JJ,Gordon KA,Chevalier RL||American journal of physiology. Renal physiology (305:F1736)||2013|
Ovarian Brenner tumour: a morphologic and immunohistochemical analysis suggesting an origin from fallopian tube epithelium.
71-6000 was used in immunohistochemistry to identify the origin of ovarian Brenner tumour
|Kuhn E,Ayhan A,Shih IeM,Seidman JD,Kurman RJ||European journal of cancer (Oxford, England : 1990) (49:3839)||2013|
Common partner Smad-independent canonical bone morphogenetic protein signaling in the specification process of the anterior rhombic lip during cerebellum development.
71-6000 was used in immunohistochemistry to examine the functions of Smad proteins during cerebellum development
|Tong KK,Kwan KM||Molecular and cellular biology (33:1925)||2013|
An aberrant cerebellar development in mice lacking matrix metalloproteinase-3.
71-6000 was used in immunohistochemistry to report a role for MMP-3 in the histogenesis of the mouse cerebellar cortex.
|Van Hove I,Verslegers M,Buyens T,Delorme N,Lemmens K,Stroobants S,Gantois I,D'Hooge R,Moons L||Molecular neurobiology (45:17)||2012|
Kruppel-like factor 5 is required for formation and differentiation of the bladder urothelium.
71-6000 was used in immunohistochemistry to show a role for KLF5 in the formation and terminal differentiation of the urothelium.
|Bell SM,Zhang L,Mendell A,Xu Y,Haitchi HM,Lessard JL,Whitsett JA||Developmental biology (358:79)||2011|
Formation of the spinal network in zebrafish determined by domain-specific pax genes.
71-6000 was used in immunohistochemistry to use transgenic zebrafish to study Pax8 localization and function.
|Ikenaga T,Urban JM,Gebhart N,Hatta K,Kawakami K,Ono F||The Journal of comparative neurology (519:1562)||2011|
Spatiotemporal fate map of neurogenin1 (Neurog1) lineages in the mouse central nervous system.
71-6000 was used in immunohistochemistry to investigate the migration and maturation of neurogenin1 neurons in the rodent central nervous system
|Kim EJ,Hori K,Wyckoff A,Dickel LK,Koundakjian EJ,Goodrich LV,Johnson JE||The Journal of comparative neurology (519:1355)||2011|
Identification of cerebellin2 in chick and its preferential expression by subsets of developing sensory neurons and their targets in the dorsal horn.
71-6000 was used in immunohistochemistry to identify cerebellin2 in chick and study its expression pattern
|Yang M,Cagle MC,Honig MG||The Journal of comparative neurology (518:2818)||2010|
Cdk5 selectively affects the migration of different populations of neurons in the developing spinal cord.
71-6000 was used in immunohistochemistry to exmaine the role of Cdk5 in the neurnal migration in the developing spinal cord
|Yip YP,Capriotti C,Drill E,Tsai LH,Yip JW||The Journal of comparative neurology (503:297)||2007|
|Mouse||Not Cited||Tlx3 and Tlx1 are post-mitotic selector genes determining glutamatergic over GABAergic cell fates.||Cheng L,Arata A,Mizuguchi R,Qian Y,Karunaratne A,Gray PA,Arata S,Shirasawa S,Bouchard M,Luo P,Chen CL,Busslinger M,Goulding M,Onimaru H,Ma Q||Nature neuroscience (7:510)||2004|
||Otx2 cell-autonomously determines dorsal mesencephalon versus cerebellum fate independently of isthmic organizing activity.||Di Giovannantonio LG,Di Salvio M,Omodei D,Prakash N,Wurst W,Pierani A,Acampora D,Simeone A||Development (Cambridge, England) (141:377)||2014|
|Mouse||1:200||The reaper-binding protein scythe modulates apoptosis and proliferation during mammalian development.||Desmots F,Russell HR,Lee Y,Boyd K,McKinnon PJ||Molecular and cellular biology (25:10329)||2005|
|Mouse||Not Cited||FKBP8 is a negative regulator of mouse sonic hedgehog signaling in neural tissues.||Bulgakov OV,Eggenschwiler JT,Hong DH,Anderson KV,Li T||Development (Cambridge, England) (131:2149)||2004|
|Mouse||Not Cited||In vitro development of mouse embryonic stem cells lacking JNK/stress-activated protein kinase-associated protein 1 (JSAP1) scaffold protein revealed its requirement during early embryonic neurogenesis.||Xu P,Yoshioka K,Yoshimura D,Tominaga Y,Nishioka T,Ito M,Nakabeppu Y||The Journal of biological chemistry (278:48422)||2003|
A transcription factor code defines nine sensory interneuron subtypes in the mechanosensory area of the spinal cord.
71-6000 was used in immunohistochemistry - frozen section to describe a combinatorial transcription factor code that defines nine different inhibitory and excitatory interneuron populations in laminae III-IV of the postnatal cord.
|Del Barrio MG,Bourane S,Grossmann K,Schüle R,Britsch S,O'Leary DD,Goulding M||PloS one (8:null)||2013|
Characterization of inducible models of Tay-Sachs and related disease.
71-6000 was used in immunohistochemistry - frozen section to investigate cellular pathogenesis of GM2 gangliosidosis.
|Sargeant TJ,Drage DJ,Wang S,Apostolakis AA,Cox TM,Cachón-González MB||PLoS genetics (8:null)||2012|
Renshaw cell interneuron specialization is controlled by a temporally restricted transcription factor program.
71-6000 was used in immunohistochemistry - frozen section to describe a transcriptional program that controls Renshaw cell differentiation.
|Stam FJ,Hendricks TJ,Zhang J,Geiman EJ,Francius C,Labosky PA,Clotman F,Goulding M||Development (Cambridge, England) (139:179)||2012|
Characterization of new otic enhancers of the pou3f4 gene reveal distinct signaling pathway regulation and spatio-temporal patterns.
71-6000 was used in immunohistochemistry - frozen section to characterize the spatio-temporal patterns of regulatory elements in the pou3f4 locus using zebrafish.
|Robert-Moreno À,Naranjo S,de la Calle-Mustienes E,Gómez-Skarmeta JL,Alsina B||PloS one (5:null)||2011|
PAX2 is activated by estradiol in breast cancer cells of the luminal subgroup selectively, to confer a low invasive phenotype.
71-6000 was used in western blot to study the role and regulation of PAX2 in the control of luminal breast cancer invasion.
|Beauchemin D,Lacombe C,Van Themsche C||Molecular cancer (10:null)||2012|
DNA ligase III is critical for mtDNA integrity but not Xrcc1-mediated nuclear DNA repair.
71-6000 was used in western blot to report that Lig3 is essential for mitochondrial DNA integrity but dispensable for nuclear DNA repair.
|Gao Y,Katyal S,Lee Y,Zhao J,Rehg JE,Russell HR,McKinnon PJ||Nature (471:240)||2011|
Potential biological role of transforming growth factor-beta1 in human congenital kidney malformations.
71-6000 was used in western blot to investigate the role of TGF-beta in the pathobiology of dysplastic kidneys.
|Yang SP,Woolf AS,Yuan HT,Scott RJ,Risdon RA,O'Hare MJ,Winyard PJ||The American journal of pathology (157:1633)||2000|
optic disc coloboma; paired box gene 2; paired box homeotic gene 2; paired box protein Pax-2
FSGS7; Opdc; PAPRS; Pax-2; PAX2