Invitrogen

PAX2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Reference

View all (29) PAX2 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

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PAX2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence staining of PAX2 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with PAX2 Polyclonal Antibody (Product # PA5-81235, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.

PAX2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of PAX2 in human renal carcinoma with PAX2 Polyclonal Antibody (Product # PA5-81235, 1:5,000, formalin-fixed paraffin embedded sections).

PAX2 Antibody in Western Blot (WB) — Western blot was performed using Anti-PAX2 Polyclonal Antibody (Product # PA5-81235) and a 40 to 45 kDa band corresponding to Paired box protein Pax-2 was observed across cell lines tested . Nuclear enriched extracts (50 µg lysate) of HEK-293 (Lane 1), SK-O-V3 (Lane 2), HaCaT (Lane 3), NIH:OVCAR-3 (Lane 4), MIA PaCa-2 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).

PAX2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK-293 cells were transfected with Paired box protein Pax-2 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-PAX2 Polyclonal Antibody (Product # PA5-81235). {KD}

Product Details

PA5-81235

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:2,500-1:10,000	-
Immunocytochemistry (ICC/IF)	1:300-1:10,000	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthetic peptide corresponding to the C-terminus of the Human PAX2 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A, Antigen affinity chromatography
Storage buffer	PBS
Contains	no preservative
Storage conditions	Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
RRID	AB_2788462

Product Specific Information

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Human PAX2.

Target Information

The pax genes are a family of transcription factors that are active in specific tissues during early embryonic development. Pax family members possess a DNA-binding domain encoded by the paired box. Because paired domain containing genes encode transcription factors, they are capable of executing a genetic program. Several pax genes have also been associated with developmental mutations including: pax-3, which is associated with Waardenburg syndrome, pax-6, which is associated with Aniridia, and pax-2, which is associated with Wilms tumor. During embryogenesis, Pax-2 is expressed in the developing kidney. In particular, the pax-2 gene is expressed in condensing metanephric mesenchyme and in early epithelial structures derived from mesenchyme; however, pax-2 mRNA and protein levels are rapidly down regulated as the tubular epithelium matures. Although Pax-2 is down regulated during renal epithelium maturation, Pax-2 expression persists in the undifferentiated epithelium of Wilms' tumors. Persistent expression of Pax-2 in Wilms' tumors occurs frequently and correlates with the proliferation of poorly differentiated epithelial cells in these tumors. Interestingly, expression of the Wilms' tumor suppresser protein, WT1, coincides with down-regulation of the pax-2 gene; thus, suggesting that WT1 can directly repress pax-2 transcription.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Derivation of dorsal spinal sensory interneurons from human pluripotent stem cells.

STAR protocols

Gupta S,Yamauchi K,Novitch BG,Butler SJ

PA5-81235 was used in Immunocytochemistry to develop two protocols for the development of sensory spinal interneurons using human embryonic and induced pluripotent stem cells.

Fri Mar 19 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: paired box homeotic gene 2; Paired box protein Pax-2

Gene Aliases: FSGS7; PAPRS; PAX2

UniProt ID: (Human) Q02962

Entrez Gene ID: (Human) 5076

Function(s)

Process(es)

core promoter proximal region sequence-specific DNA binding DNA binding protein binding transcription factor binding superoxide-generating NADPH oxidase activity transcription regulatory region DNA binding

PAX2 Polyclonal Antibody

Product Details

PA5-81235

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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