Invitrogen

PAX3 Polyclonal Antibody

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PAX3 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of PAX3 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PAX3 Rabbit Polyclonal Antibody (Product # 38-1801) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

PAX3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PAX3 showing staining in the nucleus of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- PAX3 Polyclonal Antibody (Product # 38-1801) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

PAX3 Antibody in Western Blot (WB) — Western blot was performed using Anti-PAX3 Polyclonal Antibody (Product # 38-1801) and a 55 kDa band corresponding to PAX3 was observed across positive cell lines (HEK-293, B16-F10, Neuro-2a) and not across negative cell lines (T-47D, MCF7,NIH/3T3) along with an uncharacterized band across all cell lines tested. Nuclear enriched extracts (30 µg lysate) of HEK-293 (Lane 1), T-47D (Lane 2), MCF7 (Lane 3), B16-F10 (Lane 4), Neuro-2a (Lane 5), NIH/3T3 (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 dilution using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).

PAX3 Antibody in Immunohistochemistry (IHC) — Figure 5 Photomicrograph of muscle sections in ischemic non-treated and ischemic treated groups by BM-MSCs stained for Pax3 and Pax7 nuclear expression. (a) The granulation tissue of the ischemic non-treated group showing negative expression of Pax3 (diaminobenzidine, original magnification, x200). (b) The granulation tissue of the ischemic non-treated group showing negative expression of Pax7 (diaminobenzidine, original magnification, x400). (c) The granulation tissue of the ischemic treated group by BM-MSCs showing cells with positive nuclear staining of Pax3 (diaminobenzidine, original magnification, x200). (d) The granulation tissue of the ischemic treated group by BM-MSCs showing cells with positive nuclear staining of Pax7 (diaminobenzidine, original magnification, x200)

Product Details

38-1801

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1000	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100	View 2 publications 2 publications
Immunohistochemistry (Frozen) (IHC (F))	Assay-Dependent	-
Immunocytochemistry (ICC/IF)	1:250	View 4 publications 4 publications
Immunoprecipitation (IP)	Assay-dependent	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	His6-tagged recombinant protein derived from a portion of the N-terminus of the mouse Pax3 sequence, encompassing the paired box and homeobox domains View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	whole serum
Contains	0.1% sodium azide
Storage conditions	-20°C
RRID	AB_2533359

Target Information

Pax 3 (Paired Box 3) is a transcription factor that is involved in development of the peripheral nervous system, melanocytes, some vascular smooth muscle, and a number of other derivatives. It regulates neurogenesis in pre-migratory neural crest cells from the dorsal neural tube, and in myogenic progenitors in the presomitic mesoderm and the hypaxial somites. Alternative splicing results in isoforms having different C-termini.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Therapeutic potential of mesenchymal stem cells for peripheral artery disease in a rat model of hindlimb ischemia.

Iranian journal of basic medical sciences

Ali AMEA,Ahmed AS,El-Yasergy DF,Abousarie MA,Elsayed RM,Mohammed YE,Mohammed RA

38-1801 was used in Immunohistochemistry (Paraffin) to elucidate the influence of bone marrow mesenchymal stem cell transplantation on angiogenesis in a rat model of unilateral peripheral vascular disease.

Tue Jun 01 00:00:00 EDT 2021

Therapeutic potential of mesenchymal stem cells for peripheral artery disease in a rat model of hindlimb ischemia.

Iranian journal of basic medical sciences

Ali AMEA,Ahmed AS,El-Yasergy DF,Abousarie MA,Elsayed RM,Mohammed YE,Mohammed RA

Tue Jun 01 00:00:00 EDT 2021

The protective role of DOT1L in UV-induced melanomagenesis.

Nature communications

Zhu B,Chen S,Wang H,Yin C,Han C,Peng C,Liu Z,Wan L,Zhang X,Zhang J,Lian CG,Ma P,Xu ZX,Prince S,Wang T,Gao X,Shi Y,Liu D,Liu M,Wei W,Wei Z,Pan J,Wang Y,Xuan Z,Hess J,Hayward NK,Goding CR,Chen X,Zhou J,Cui R

Published figure using PAX3 polyclonal antibody (Product # 38-1801) in Immunohistochemistry

Wed Jan 17 00:00:00 EST 2018

Loss of Ezh2 promotes a midbrain-to-forebrain identity switch by direct gene derepression and Wnt-dependent regulation.

BMC biology

Zemke M,Draganova K,Klug A,Schöler A,Zurkirchen L,Gay MH,Cheng P,Koseki H,Valenta T,Schübeler D,Basler K,Sommer L

38-1801 was used in immunohistochemistry - paraffin section to assess the Wnt-dependent regulation and midbrain-to-forebrain identity switch by loss of Ezh2

Mon Nov 30 00:00:00 EST 2015

Recurrent PAX3-MAML3 fusion in biphenotypic sinonasal sarcoma.

Nature genetics

Wang X,Bledsoe KL,Graham RP,Asmann YW,Viswanatha DS,Lewis JE,Lewis JT,Chou MM,Yaszemski MJ,Jen J,Westendorf JJ,Oliveira AM

38-1801 was used in western blot to investigate PAX3-MAML3 fusion in biphenotypic sinonasal sarcoma

Tue Jul 01 00:00:00 EDT 2014

SOX2-LIN28/let-7 pathway regulates proliferation and neurogenesis in neural precursors.

Proceedings of the National Academy of Sciences of the United States of America

Cimadamore F,Amador-Arjona A,Chen C,Huang CT,Terskikh AV

Tue Aug 06 00:00:00 EDT 2013

Direct reprogramming of melanocytes to neural crest stem-like cells by one defined factor.

Stem cells (Dayton, Ohio)

Zabierowski SE,Baubet V,Himes B,Li L,Fukunaga-Kalabis M,Patel S,McDaid R,Guerra M,Gimotty P,Dahmane N,Dahamne N,Herlyn M

Published figure using PAX3 polyclonal antibody (Product # 38-1801) in Western Blot

Tue Nov 01 00:00:00 EDT 2011

Direct reprogramming of melanocytes to neural crest stem-like cells by one defined factor.

Stem cells (Dayton, Ohio)

Zabierowski SE,Baubet V,Himes B,Li L,Fukunaga-Kalabis M,Patel S,McDaid R,Guerra M,Gimotty P,Dahmane N,Dahamne N,Herlyn M

Published figure using PAX3 polyclonal antibody (Product # 38-1801) in Western Blot

Tue Nov 01 00:00:00 EDT 2011

Pax3-FKHR knock-in mice show developmental aberrations but do not develop tumors.

Molecular and cellular biology

Lagutina I,Conway SJ,Sublett J,Grosveld GC

Tue Oct 01 00:00:00 EDT 2002

Pax3-FKHR knock-in mice show developmental aberrations but do not develop tumors.

Molecular and cellular biology

Lagutina I,Conway SJ,Sublett J,Grosveld GC

Tue Oct 01 00:00:00 EDT 2002

The EF-hand calcium-binding protein calmyrin inhibits the transcriptional and DNA-binding activity of Pax3.

Biochimica et biophysica acta

Hollenbach AD,McPherson CJ,Lagutina I,Grosveld G

Fri Apr 12 00:00:00 EDT 2002

The oncogenic potential of the Pax3-FKHR fusion protein requires the Pax3 homeodomain recognition helix but not the Pax3 paired-box DNA binding domain.

Molecular and cellular biology

Lam PY,Sublett JE,Hollenbach AD,Roussel MF

Fri Jan 01 00:00:00 EST 1999

The oncogenic potential of the Pax3-FKHR fusion protein requires the Pax3 homeodomain recognition helix but not the Pax3 paired-box DNA binding domain.

Molecular and cellular biology

Lam PY,Sublett JE,Hollenbach AD,Roussel MF

Fri Jan 01 00:00:00 EST 1999

Bioinformatics

Protein Aliases: HuP2; MGC120381; MGC120382; MGC120383; MGC120384; MGC134778; paired box gene 3; paired box homeotic gene 3; Paired box protein Pax-3; paired domain gene 3; paired domain gene HuP2; PAX3/FKHR fusion

Gene Aliases: CDHS; HUP2; Pax-3; PAX3; Sp; splotch; WS1; WS3

UniProt ID: (Human) P23760, (Mouse) P24610

Entrez Gene ID: (Human) 5077, (Mouse) 18505

Function(s)