Invitrogen

PAX3 Polyclonal Antibody

Product Image Gallery

PAX3 Antibody — Published figure using PAX3 polyclonal antibody (Product # 38-1801)

PAX3 Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of PAX3 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PAX3 Rabbit Polyclonal Antibody (Product # 38-1801) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

PAX3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PAX3 showing staining in the nucleus of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- PAX3 Polyclonal Antibody (Product # 38-1801) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

PAX3 Antibody in Western Blot (WB) — Western blot analysis of Pax3-MSCV transduced NIH 3T3 cell lysates using Pax3 Polyclonal Antibody, Rabbit (Product # 38-1801).

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:250

Immunofluorescence (IF)

1:250

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunoprecipitation (IP)

Assay Dependent

Western Blot (WB)

Assay Dependent

Published Applications

Immunohistochemistry (IHC)

See 2 publications below

Immunohistochemistry (Paraffin) (IHC (P))

See 1 publication below

Immunofluorescence (IF)

See 4 publications below

Western Blot (WB)

See 2 publications below

Immunocytochemistry (ICC)

See 1 publication below

Immunoprecipitation (IP)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

His6-tagged recombinant protein derived from a portion of the N-terminus of the mouse Pax3 sequence, encompassing the paired box and homeobox domains

Conjugate

Unconjugated

Form

Liquid

Storage buffer

whole serum

Contains

0.1% sodium azide

Storage conditions

-20°C

RRID

AB_2533359

Target Information

Pax 3 (Paired Box 3) is a transcription factor that is involved in development of the peripheral nervous system, melanocytes, some vascular smooth muscle, and a number of other derivatives. It regulates neurogenesis in pre-migratory neural crest cells from the dorsal neural tube, and in myogenic progenitors in the presomitic mesoderm and the hypaxial somites. Alternative splicing results in isoforms having different C-termini.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CDHS; HUP2; paired box gene 3; paired box homeotic gene 3; Paired box protein Pax-3; paired domain gene 3; paired domain gene HuP2; PAX3/FKHR fusion; WS1; WS3

Gene Aliases: CDHS; HUP2; Pax-3; PAX3; Sp; splotch; WS1; WS3

UniProt ID: (Human) P23760, (Mouse) P24610

Entrez Gene ID: (Human) 5077, (Mouse) 18505

Molecular Function: DNA binding protein helix-turn-helix transcription factor homeobox transcription factor nucleic acid binding transcription factor