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Immunofluorescence analysis of PAX3 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PAX3 Rabbit Polyclonal Antibody (381801) at 2 ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||His6-tagged recombinant protein derived from a portion of the N-terminus of the mouse Pax3 sequence, encompassing the paired box and homeobox domains|
|Storage buffer||whole serum|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2-3 µg/ml|
|Immunofluorescence (IF)||2-3 µg/ml|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Pax 3 (Paired Box 3) is a transcription factor that is involved in development of the peripheral nervous system, melanocytes, some vascular smooth muscle, and a number of other derivatives. It regulates neurogenesis in pre-migratory neural crest cells from the dorsal neural tube, and in myogenic progenitors in the presomitic mesoderm and the hypaxial somites. Alternative splicing results in isoforms having different C-termini.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Recurrent PAX3-MAML3 fusion in biphenotypic sinonasal sarcoma.
38-1801 was used in western blot to investigate PAX3-MAML3 fusion in biphenotypic sinonasal sarcoma
|Wang X,Bledsoe KL,Graham RP,Asmann YW,Viswanatha DS,Lewis JE,Lewis JT,Chou MM,Yaszemski MJ,Jen J,Westendorf JJ,Oliveira AM||Nature genetics (46:666)||2014|
|Human||1:200||SOX2-LIN28/let-7 pathway regulates proliferation and neurogenesis in neural precursors.||Cimadamore F,Amador-Arjona A,Chen C,Huang CT,Terskikh AV||Proceedings of the National Academy of Sciences of the United States of America (110:E3017)||2013|
|Pax3-FKHR knock-in mice show developmental aberrations but do not develop tumors.||Lagutina I,Conway SJ,Sublett J,Grosveld GC||Molecular and cellular biology (22:7204)||2002|
|Mouse||Not Cited||The EF-hand calcium-binding protein calmyrin inhibits the transcriptional and DNA-binding activity of Pax3.||Hollenbach AD,McPherson CJ,Lagutina I,Grosveld G||Biochimica et biophysica acta (1574:321)||2002|
||The oncogenic potential of the Pax3-FKHR fusion protein requires the Pax3 homeodomain recognition helix but not the Pax3 paired-box DNA binding domain.||Lam PY,Sublett JE,Hollenbach AD,Roussel MF||Molecular and cellular biology (19:594)||1999|
|Mouse||Not Cited||The oncogenic potential of the Pax3-FKHR fusion protein requires the Pax3 homeodomain recognition helix but not the Pax3 paired-box DNA binding domain.||Lam PY,Sublett JE,Hollenbach AD,Roussel MF||Molecular and cellular biology (19:594)||1999|
CDHS; HUP2; paired box gene 3; paired box homeotic gene 3; paired box protein Pax-3; paired domain gene 3; paired domain gene HuP2; PAX3/FKHR fusion; WS1; WS3
CDHS; HUP2; Pax-3; PAX3; Sp; splotch; WS1; WS3