Immunofluorescence was performed on adult human pancreas tissue. To expose target proteins, antigen retrieval was performed by heating tissues at 95C for 15 minutes in 10mM citrate buffer, pH 6.0. Following antigen retrieval, tissues were blocked and then probed with a Pax4 polyclonal antibody (Product # PA1-108) at a dilution of 1:500 overnight at 4C. Tissues were washed extensively and detection was performed using a fluorescently-conjugated anti-rabbit IgG secondary antibody (green). Tissues were also stained with an antibody against insulin (red), and nuclei (grey) were stained with DAPI. Higher magnification of regions inside the white boxes (left panel) are shown to the right. Scale bar = 200um. Data supplied courtesy of the Innovators Program.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Amino acids 128-350 of human PAX4|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-108 has been validated for WB on recombinant human Pax4 protein, but may not be suitable for detecting endogenous Pax4.
By immunofluorescence, PA1-108 has been used to stain paraffin-embedded human and mouse pancreatic tissue.
This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The paired box 4 gene is involved in pancreatic islet development and mouse studies have demonstrated a role for this gene in differentiation of insulin-producing beta cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.