Invitrogen

PAX4 Polyclonal Antibody

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Antibody Testing Data (3)
Published Figures (2)

PAX4 Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 5

PAX4 Antibody (PA1-108) in IHC

Immunofluorescence was performed on adult mouse pancreas tissue. To expose target proteins, antigen retrieval was performed by heating tissues at 95C for 15 minutes in 10mM citrate buffer, pH 6.0. Following antigen retrieval, tissues were blocked and then probed with a Pax4 polyclonal antibody (Product # PA1-108) at a dilution of 1:500 overnight at 4C. Tissues were washed extensively and detection was performed using a fluorescently-conjugated anti-rabbit IgG secondary antibody (green). Tissues were also stained with an antibody against in... View More

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PAX4 Antibody in Western Blot (WB) — Western blot analysis of Pax4 was performed by loading the indicated amounts of recombinant Pax3 (negative control) or Pax4 proteins, and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a Novex® 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter (Product # 62288), and blocked with 5% milk in TBST for at least 1 hour at room temperature. Pax4 was detected at ~38 kD using a Pax4 polyclonal antibody (Product # PA1-108) at a dilution of 1:1000 in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugate goat anti-rabbit IgG secondary antibody (Product # 31460) at a dilution of 1:40,000 for at least 30 minutes. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080). NOTE: The presence of Pax3 recombinant protein was determined by probing a duplicate blot with a Pax3 polyclonal antibody (Product # PA1-107), and the corresponding image is on the webpage for Product # PA1-107.

PAX4 Antibody in Immunocytochemistry (ICC/IF) — Fig. 2 Stage wise Immunohistochemistry of the hiPSC cells undergoing differentiation. Foregut lineage cells show positive staining for PDX1 and faint staining for few progenitor markers. Pancreatic progenitor cells show positive staining for all the stage specific markers

PAX4 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Figure 1 Elevated PAX4 mRNA and protein expression in both GC tissues and cell lines. ( A , B ) Higher PAX4 mRNA and protein expression in GC tissues compared to normal tissues ( n = 60) by qRT-PCR ( P < 0.0001) and WB ( P = 0.0002). ( C ) The IHC results between GC and non-GC tissues demonstrated excessive PAX4 in GC tissues. ( D ) Overexpression of PAX4 mRNA levels were identified across six GC cell lines (HGC-27, MGC803, BGC-823, SGC-7901, AGS, MKN45) compared to the human gastric mucosal epithelial cell line GES-1. ( E ) The overall survival analysis results demonstrated that PAX4 high expression in GC is associated with poor prognosis.

Product Details

PA1-108

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

1:500

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Amino acids 128-350 of human PAX4

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

RRID

AB_2539882

Product Specific Information

PA1-108 has been validated for WB on recombinant human Pax4 protein, but may not be suitable for detecting endogenous Pax4.

By immunofluorescence, PA1-108 has been used to stain paraffin-embedded human and mouse pancreatic tissue.

Target Information

This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The paired box 4 gene is involved in pancreatic islet development and mouse studies have demonstrated a role for this gene in differentiation of insulin-producing beta cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Transcription factor PAX4 facilitates gastric cancer progression through interacting with miR-27b-3p/Grb2 axis.

Aging

Zhang Y,Ding L,Ni Q,Tao R,Qin J

Published figure using PAX4 polyclonal antibody (Product # PA1-108) in Western Blot

Wed Jun 23 00:00:00 EDT 2021

Transcription factor PAX4 facilitates gastric cancer progression through interacting with miR-27b-3p/Grb2 axis.

Aging

Zhang Y,Ding L,Ni Q,Tao R,Qin J

Published figure using PAX4 polyclonal antibody (Product # PA1-108) in Western Blot

Wed Jun 23 00:00:00 EDT 2021

Transcription factor PAX4 facilitates gastric cancer progression through interacting with miR-27b-3p/Grb2 axis.

Aging

Zhang Y,Ding L,Ni Q,Tao R,Qin J

Published figure using PAX4 polyclonal antibody (Product # PA1-108) in Western Blot

Wed Jun 23 00:00:00 EDT 2021

Transcription factor PAX4 facilitates gastric cancer progression through interacting with miR-27b-3p/Grb2 axis.

Aging

Zhang Y,Ding L,Ni Q,Tao R,Qin J

Published figure using PAX4 polyclonal antibody (Product # PA1-108) in Western Blot

Wed Jun 23 00:00:00 EDT 2021

Development of islet organoids from human induced pluripotent stem cells in a cross-linked collagen scaffold.

Cell regeneration (London, England)

Sandilya S,Singh S

Published figure using PAX4 polyclonal antibody (Product # PA1-108) in Immunocytochemistry

Wed Dec 01 00:00:00 EST 2021

Expansion of transplanted islets in mice by co-transplantation with adipose tissue-derived mesenchymal stem cells.

Heliyon

Tanaka T,Kojima D,Mera T,Matsumoto M,Yasunami Y,Yanase T

PA1-108 was used in Immunohistochemistry to demonstrate that co-transplantation with adipose tissue-derived mesenchymal stem cells induces proliferation of transplanted islets in mice.

Tue May 01 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: MGC129960; paired box gene 4; Paired box protein Pax-4; paired domain gene 4

Gene Aliases: KPD; MODY9; Pax-4; Pax4

UniProt ID: (Mouse) P97341

Entrez Gene ID: (Human) 5078, (Mouse) 18506

Function(s)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated circadian rhythm organ morphogenesis cell differentiation endocrine pancreas development response to drug negative regulation of apoptotic process positive regulation of cell differentiation response to cAMP retina development in camera-type eye regulation of transcription, DNA-templated transcription from RNA polymerase II promoter multicellular organism development regulation of cell differentiation negative regulation of transcription, DNA-templated

Process(es)

RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II distal enhancer sequence-specific binding DNA binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding

PAX4 Polyclonal Antibody

PAX4 Antibody (PA1-108) in IHC

Product Details

PA1-108

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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