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Invitrogen
PAX5 Polyclonal Antibody
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FIGURE: 1 / 15
PAX5 Antibody (PA1-109) in ICC/IF
Immunofluorescent analysis of PAX5 (green) in Ramos and negative control Jurkat cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 2% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a PAX5 polyclonal antibody (Product # PA1-109) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody (Product # 35552) at a d... View More
Product Details
PA1-109
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Purified internal fragment of human recombinant PAX5 expressed in E. coli
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA, 30% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Western blot analysis of PA1-109 detects a prominent ~45 kDa protein in PAX5 expressing human Ramos, Daudi, Gaji and mouse A20 B-cell lymphoma cells. No PAX5 was detected in negative control Jurkat cells. The 45 kDa band is enriched in immunoprecipitations from Raji lysate, however additional unknown high molecular weight bands are also enriched and detected by PA1-109. PAX5 was also specifically detected by immunofluorescence in the nuclei of Ramos cells compared to negative control Jurkat cells.
Target Information
PAX5 is a member of the paired box (PAX) family of transcription factors. The PAX proteins are important regulators in early development and alterations in the expression of their genes are thought to contribute to neoplastic transformation. PAX5 is the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages, of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX5 may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis. Mutations in the gene can result in leukemia, acute lymphoblastic 3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B-cell lineage specific activator; B-cell-specific transcription factor; BSAP; BSAP; transcription factor; paired box gene 5; paired box homeotic gene 5; Paired box protein Pax-5; paired domain gene 5; Pax-5; Pax5 variant 1; Pax5 variant 2; transcription factor PAX 5
Gene Aliases: ALL3; BSAP; EBB-1; KLP; PAX-5; PAX5
UniProt ID: (Human) Q02548, (Mouse) Q02650
Entrez Gene ID: (Human) 5079, (Mouse) 18507
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
multicellular organism development
spermatogenesis
nervous system development
aging
organ morphogenesis
lateral ventricle development
cerebral cortex development
cell differentiation
adult behavior
skeletal muscle cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
embryonic cranial skeleton morphogenesis
negative regulation of histone H3-K9 methylation
regulation of transcription from RNA polymerase II promoter
sensory organ development
B cell differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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