|Flow Cytometry (Flow)||1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:500|
|Immunoprecipitation (IP)||2-5 µg|
|Western Blot (WB)||1:500-1:2000|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified internal fragment of human recombinant PAX5 expressed in E. coli|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
Western blot analysis of PA1-109 detects a prominent ~45 kDa protein in PAX5 expressing human Ramos, Daudi, Gaji and mouse A20 B-cell lymphoma cells. No PAX5 was detected in negative control Jurkat cells. The 45 kDa band is enriched in immunoprecipitations from Raji lysate, however additional unknown high molecular weight bands are also enriched and detected by PA1-109. PAX5 was also specifically detected by immunofluorescence in the nuclei of Ramos cells compared to negative control Jurkat cells.
PAX5 is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. PAX5 is expressed during B-lymphopoiesis and plays an essential role in early B, pre-B and pro-B lymphocyte development. PAX5 is also expressed in B-cell lymphomas, in developing CNS and testis and so the
encoded protein may also play a role in neural development and spermatogenesis. The PAX5 gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. The translocation brings the E-mu enhancer of the IgH gene into close proximity to the PAX5 promoter, suggesting that the deregulation of transcription of PAX5 may contribute to the pathogenesis of these lymphomas. In constrast, a tumor suppressor role for PAX5 has been suggested in hepatocellular carcinoma and overexpression of PAX5 resulted in apoptosis in multiple myeloma.
PAX5 expression was also found in a subset of breast cancer cells and has been associated with better prognosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B Cell Specific Activator Protein; B-Cell Lineage Specific Activator; B-Cell-Specific Transcription Factor; BSAP; paired box 5; paired box gene 5; Paired Box Gene 5 (B-Cell Lineage Specific Activator Protein); paired box homeotic gene 5; Paired Box Protein Pax-5; paired domain gene 5; Pax5 variant 1; Pax5 variant 2; Transcription Factor PAX 5
Gene Aliases: ALL3; BSAP; EBB-1; KLP; Pax-5; PAX5
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