This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details
Western blot analysis of PA1-109 detects a prominent ~45 kDa protein in PAX5 expressing human Ramos, Daudi, Gaji and mouse A20 B-cell lymphoma cells. No PAX5 was detected in negative control Jurkat cells. The 45 kDa band is enriched in immunoprecipitations from Raji lysate, however additional unknown high molecular weight bands are also enriched and detected by PA1-109. PAX5 was also specifically detected by immunofluorescence in the nuclei of Ramos cells compared to negative control Jurkat cells.
PAX5 is a member of the paired box (PAX) family of transcription factors. The PAX proteins are important regulators in early development and alterations in the expression of their genes are thought to contribute to neoplastic transformation. PAX5 is the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages, of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX5 may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis. Mutations in the gene can result in leukemia, acute lymphoblastic 3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B Cell Specific Activator Protein; B-Cell Lineage Specific Activator; B-Cell-Specific Transcription Factor; BSAP; paired box 5; paired box gene 5; Paired Box Gene 5 (B-Cell Lineage Specific Activator Protein); paired box homeotic gene 5; Paired Box Protein Pax-5; paired domain gene 5; Pax5 variant 1; Pax5 variant 2; Transcription Factor PAX 5
Gene Aliases: ALL3; BSAP; EBB-1; KLP; Pax-5; PAX5