Invitrogen

PAX7 Polyclonal Antibody

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PAX7 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of PAX7 was performed using 70% confluent log phase A-673 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PAX7 Polyclonal Antibody (Product # PA1-117) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained withRhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

PAX7 Antibody in Western Blot (WB) — Western blot analysis of PAX7 was performed by loading 50 µg of various whole cell lysates and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a PAX7 polyclonal antibody (Product # PA1-117) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-rabbit HRP secondary antibody (Product # 31460) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075). Note: PAX7 was detected in whole cell lysates from HeLa, MCF7, in the PAX7 positive control Rhabdomyosarcoma cell line SJCRH30, and in primary Human Skeletal Muscle Satellite Cells. In HeLa, SJCRH30, and HSkMSC lysates the antibody detected both PAX7 isoforms (PAX7-A and PAX7-B).

PAX7 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of PAX7 was performed using HeLa whole cell lysate. Antigen-antibody complexes were formed by incubating 500 µg of lysate with 3 µg of a PAX7 polyclonal antibody (Product # PA1-117) overnight on a rocking platform at 4°C. The immune complexes were captured on 50 µL Protein A/G Agarose (Product # 20421), washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer (Product # 39000). Samples was resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween-20 for 1 hour. The membrane was probed with a PAX7 polyclonal antibody (Product # PA1-117) at a dilution of 1:1000 overnight rotating at 4°C. Membranes were washed in TBST, and probed with Clean-Blot IP Detection Reagent (Product # 21230) at a dilution of 1:1000 for at least one hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).

PAX7 Antibody in Immunohistochemistry (IHC) — Figure 6 Enhanced SC numbers but no changes in growth factor mRNA levels after FN injury in SRF deficient animals. ( A-C ) Masseter muscles at 21 dpi were stained for the SC marker Pax7 (green), and DAPI (blue). Pax7 abundance was elevated in SRF depleted muscles ( B ) compared to wt myofibers. (A'',B'') show the Pax7 channel of wt ( A ) and Srf mutant ( B ) only. Quantification of Pax7 positive cells/area ( C ). ( D-I ) cDNA of uninjured and injured masseter muscles of wt and Srf loxp/loxp : HSA-CreERT2 mutant animals (ko) was subjected to qPCR analysis for genes indicated at 7 dpi. Ptgs2 ( D ), Igf2 ( E ) were strongly and Gdnf ( F ) and Ngf ( G ) were mildly upregulated by injury with no differences between genotypes. Vegfb ( H ) and Bdnf ( I ) were downregulated by injury in a comparable manner between wt and ko animals. Each black circle in a bar reflects one animal analyzed. Data were provided as mean +- SD. *P < 0.05, **P < 0.001, ***P < 0.001. Scale-bar ( A , B ) = 30 mum.

Product Details

PA1-117

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:2,000	View 2 publications 2 publications
Immunocytochemistry (ICC/IF)	1:20-1:200	View 2 publications 2 publications
Immunoprecipitation (IP)	3 µg	-
Immunohistochemistry (IHC)	-	View 5 publications 5 publications
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Purified internal fragment of human recombinant PAX7 expressed in E. coli.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS with 30% glycerol, 1mg/mL BSA
Contains	0.05% sodium azide
Storage conditions	-20°C
RRID	AB_2539886

Product Specific Information

Western blot analysis of PA1-117 detects a prominent ~54 kDa protein in human HeLa, MCF7, rhabdomyosarcoma and skeletal muscle satellite cells and a weaker ~57 kDa protein in HeLa, rhabdomyosarcoma and skeletal muscle satellite cells. The ~57 kDa band seems to be enriched in immunoprecipitations from HeLa lysate, suggesting a bias for the larger isoform in ths application. Weak unknown bands of lower molecular weight and at ~110-130 kDa are also detected. Specificity of the antibody was also confirmed in Hela cells overexpressing full length PAX7.

Target Information

This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Three transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Uhrf1 governs the proliferation and differentiation of muscle satellite cells.

iScience

Sakai H,Sawada Y,Tokunaga N,Tanaka K,Nakagawa S,Sakakibara I,Ono Y,Fukada SI,Ohkawa Y,Kikugawa T,Saika T,Imai Y

PA1-117 was used in Immunohistochemistry to show that Uhrf1 is a critical epigenetic regulator of proliferation and differentiation in satellite cells, by controlling cell-type-specific gene expression via maintenance of DNA methylation.

Fri Mar 18 00:00:00 EDT 2022

G-quadruplex DNA structures in human stem cells and differentiation.

Nature communications

Zyner KG,Simeone A,Flynn SM,Doyle C,Marsico G,Adhikari S,Portella G,Tannahill D,Balasubramanian S

PA1-117 was used in Immunocytochemistry to determine whether G-quadruplexes (G4s) are key genomic structural features linked to cellular differentiation.

Mon Jan 10 00:00:00 EST 2022

Meflin defines mesenchymal stem cells and/or their early progenitors with multilineage differentiation capacity.

Genes to cells : devoted to molecular & cellular mechanisms

Hara A,Kato K,Ishihara T,Kobayashi H,Asai N,Mii S,Shiraki Y,Miyai Y,Ando R,Mizutani Y,Iida T,Takefuji M,Murohara T,Takahashi M,Enomoto A

PA1-117 was used in Immunohistochemistry to investigate if Meflin is a useful potential marker to localize Mesenchymal stem cells and/or their immature progenitors in multiple tissues.

Thu Jul 01 00:00:00 EDT 2021

Dysregulation of Muscle-Specific MicroRNAs as Common Pathogenic Feature Associated with Muscle Atrophy in ALS, SMA and SBMA: Evidence from Animal Models and Human Patients.

International journal of molecular sciences

Malacarne C,Galbiati M,Giagnorio E,Cavalcante P,Salerno F,Andreetta F,Cagnoli C,Taiana M,Nizzardo M,Corti S,Pensato V,Venerando A,Gellera C,Fenu S,Pareyson D,Masson R,Maggi L,Dalla Bella E,Lauria G,Mantegazza R,Bernasconi P,Poletti A,Bonanno S,Marcuzzo S

Published figure using PAX7 polyclonal antibody (Product # PA1-117) in Western Blot

Wed May 26 00:00:00 EDT 2021

Interference with SRF expression in skeletal muscles reduces peripheral nerve regeneration in mice.

Scientific reports

Wanner R,Knöll B

PA1-117 was used in Immunohistochemistry to conditionally ablated SRF (serum response factor), an important myofiber transcription factor, in skeletal muscles of mice.

Tue Mar 24 00:00:00 EDT 2020

Interference with SRF expression in skeletal muscles reduces peripheral nerve regeneration in mice.

Scientific reports

Wanner R,Knöll B

PA1-117 was used in Immunohistochemistry to conditionally ablated SRF (serum response factor), an important myofiber transcription factor, in skeletal muscles of mice.

Tue Mar 24 00:00:00 EDT 2020

Histone H3.3 sub-variant H3mm7 is required for normal skeletal muscle regeneration.

Nature communications

Harada A,Maehara K,Ono Y,Taguchi H,Yoshioka K,Kitajima Y,Xie Y,Sato Y,Iwasaki T,Nogami J,Okada S,Komatsu T,Semba Y,Takemoto T,Kimura H,Kurumizaka H,Ohkawa Y

PA1-117 was used in Immunohistochemistry to characterise the function of H3mm7, a histone H3.3 sub-variant expressed in skeletal muscle cells.

Wed Apr 11 00:00:00 EDT 2018

Age-dependent increase in angiopoietin-like protein 2 accelerates skeletal muscle loss in mice.

The Journal of biological chemistry

Zhao J,Tian Z,Kadomatsu T,Xie P,Miyata K,Sugizaki T,Endo M,Zhu S,Fan H,Horiguchi H,Morinaga J,Terada K,Yoshizawa T,Yamagata K,Oike Y

PA1-117 was used in Immunohistochemistry-immunofluorescence to aNGPTL2 up-regulation in skeletal myocytes accelerates muscle atrophy, and exercise-induced attenuation of ANGPTL2 expression in those tissues may partially explain how exercise training prevents sarcopenia.

Fri Feb 02 00:00:00 EST 2018

Age-dependent increase in angiopoietin-like protein 2 accelerates skeletal muscle loss in mice.

The Journal of biological chemistry

Zhao J,Tian Z,Kadomatsu T,Xie P,Miyata K,Sugizaki T,Endo M,Zhu S,Fan H,Horiguchi H,Morinaga J,Terada K,Yoshizawa T,Yamagata K,Oike Y

Fri Feb 02 00:00:00 EST 2018

Efficient differentiation of human pluripotent stem cells into skeletal muscle cells by combining RNA-based MYOD1-expression and POU5F1-silencing.

Scientific reports

Akiyama T,Sato S,Chikazawa-Nohtomi N,Soma A,Kimura H,Wakabayashi S,Ko SBH,Ko MSH

PA1-117 was used in Western Blotting to show a rapid and robust method to induce myogenic differentiation of human pluripotent stem cells by introducing mRNA encoding MYOD1 together with siRNA-mediated knockdown of POU5F1.

Fri Jan 19 00:00:00 EST 2018

Targeting β1-integrin signaling enhances regeneration in aged and dystrophic muscle in mice.

Nature medicine

Rozo M,Li L,Fan CM

Published figure using PAX7 polyclonal antibody (Product # PA1-117) in Immunofluorescence

Mon Aug 01 00:00:00 EDT 2016

Enobosarm (GTx-024) Modulates Adult Skeletal Muscle Mass Independently of the Androgen Receptor in the Satellite Cell Lineage.

Endocrinology

Dubois V,Simitsidellis I,Laurent MR,Jardi F,Saunders PT,Vanderschueren D,Claessens F

PA1-117 was used in immunohistochemistry - paraffin section to study the role of muscle androgen receptor in GTx-024's anabolic effect

Tue Dec 01 00:00:00 EST 2015

Bioinformatics

Protein Aliases: FLJ37460; HuP1; paired box gene 7; paired box homeotic gene 7; Paired box protein Pax-7; paired box transcription factor PAX7; paired domain gene 7; PAX7 transcriptional factor; PAX7A

Gene Aliases: HUP1; Pax-7; PAX7; PAX7B; RMS2

UniProt ID: (Human) P23759, (Mouse) P47239

Entrez Gene ID: (Human) 5081, (Mouse) 18509

Function(s)

chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter multicellular organism development muscle organ development skeletal muscle tissue development organ morphogenesis regulation of cell fate commitment regulation of gene expression skeletal muscle satellite cell commitment spinal cord association neuron differentiation dorsal/ventral neural tube patterning positive regulation of histone methylation regulation of protein binding skeletal muscle tissue regeneration positive regulation of transcription from RNA polymerase II promoter neuron fate commitment embryonic skeletal system development cartilage development muscle tissue morphogenesis positive regulation of myoblast proliferation anatomical structure morphogenesis negative regulation of apoptotic process

Process(es)

transcription factor activity, RNA polymerase II core promoter sequence-specific transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding DNA binding protein binding

PAX7 Polyclonal Antibody

Product Details

PA1-117

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Immunohistochemistry (IHC)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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