This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details
Western blot analysis of PA1-112 detects a prominent ~48 kDa protein in human adenocarcinoma SKOV-3 and SV40 transformed African green monkey (Cercopithecus aethiops) kidney cells. In SKOV-3 cells, 2 additional unknown bands at (~20 kD) and at ~150 kDa are also detected. Specificity of the antibody was confirmed in HeLa and 293T cells overexpressing full lenght PAX8. The 48 kDa band is enriched in immunoprecipitations from Cos7 lysate, however additional unknown high molecular weight bands are also enriched and detected by PA1-112.
This gene encodes a member of the paired box family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described.
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Protein Aliases: HGNC:8622; Paired box protein Pax-8; paired domain gene 8
Gene Aliases: PAX8
UniProt ID: (Human) Q06710
Entrez Gene ID: (Human) 7849