|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:500|
|Immunoprecipitation (IP)||2-5 µg|
|Western Blot (WB)||1:500-1:2000|
|Tested Species reactivity||Human, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified internal fragment of human recombinant PAX8 expressed in E. coli|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
Western blot analysis of PA1-112 detects a prominent ~48 kDa protein in human adenocarcinoma SKOV-3 and SV40 transformed African green monkey (Cercopithecus aethiops) kidney cells. In SKOV-3 cells, 2 additional unknown bands at (~20 kD) and at ~150 kDa are also detected. Specificity of the antibody was confirmed in HeLa and 293T cells overexpressing full lenght PAX8. The 48 kDa band is enriched in immunoprecipitations from Cos7 lysate, however additional unknown high molecular weight bands are also enriched and detected by PA1-112.
PAX8 is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. Pax8 is expressed in the developing and adult thyroid and kidney. In the thyroid, PAX8 regulates thyroglobulin, thyroidperoxidase,and NIS (sodium/iodide symporter) expression. Mutations in PAX8 have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: paired box 8; paired box homeotic gene 8; Paired Box Protein Pax-8; Paired Domain Gene 8
Gene Aliases: PAX8
UniProt ID: (Human) Q06710
Entrez Gene ID: (Human) 7849
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