Active Motif

PBRM1 Polyclonal Antibody

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PBRM1 Antibody in Western Blot (WB) — PBRM1 antibody (pAb) tested by Western blot. Detection of PBRM1 by Western blot. The analysis was performed using 40 µg A-431 nuclear extract and PBRM1 (pAb) at a 1:500 dilution.

PBRM1 Antibody in ChIP-sequencing (ChIP-Seq) — PBRM1 antibody (pAb) tested by ChIP-Seq. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from mouse liver and 4 µg PBRM1 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 3.9 million sequence tags were mapped to identify PBRM1 binding sites.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

ChIP-sequencing (ChIP-Seq)

4 µg

Product Specifications

Species

Human, Mouse

Expression System

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This antibody was raised against a peptide within the N-terminal region of human PBRM1.

Conjugate

Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.5, with 30% glycerol

Contains

0.035% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2793612

Target Information

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Cite this antibody

Bioinformatics

Protein Aliases: 2310032M22Rik; BAF180; BRG1-associated factor 180; hPB1; Polybromo-1D; Protein polybromo-1

Gene Aliases: 2610016F04Rik; AI507524; BAF180; PB1; PBRM1

UniProt ID: (Human) Q86U86

Entrez Gene ID: (Human) 55193, (Mouse) 66923

Function(s)

chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated mitotic nuclear division negative regulation of cell proliferation covalent chromatin modification placenta development blood vessel remodeling heart morphogenesis epicardium-derived cardiac endothelial cell differentiation heart development chromatin modification positive regulation of transcription, DNA-templated cardiac vascular smooth muscle cell development vasculogenesis involved in coronary vascular morphogenesis

Process(es)

DNA binding chromatin binding protein binding

PBRM1 Polyclonal Antibody

Product Details

Target Information

References (0)

Bioinformatics

Material safety data sheets (MSDS)

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