Bethyl Laboratories

PBRM1 Polyclonal Antibody

View all (15) PBRM1 antibodies

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Questions & Answers

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PBRM1 Antibody in Immunohistochemistry (IHC) — Detection of human PBRM1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PBRM1(Cat. No. A301-591A Lot4) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

PBRM1 Antibody in Immunohistochemistry (IHC) — Detection of mouse PBRM1 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- PBRM1 (Cat. No. A301-591A Lot4) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

PBRM1 Antibody in Western Blot (WB) — Detection of human PBRM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PBRM1 antibody A301-591A (lot A301-591A-4) used for IP at 3 µg per reaction. PBRM1 was also immunoprecipitated by rabbit anti-PBRM1 antibody A301-590A. For blotting immunoprecipitated PBRM1, A301-591A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

PBRM1 Antibody in Western Blot (WB) — Detection of human and mouse PBRM1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBRM1 antibody A301-591A (lot A301-591A-4) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Product Details

A301-591A

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000-1:10,000	-
Immunohistochemistry (IHC)	1:500-1:2,000	-
Immunoprecipitation (IP)	2-5 µg/mg lysate	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Between 1639 and 1689
Conjugate	Unconjugated
Form	liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	tris citrate/phosphate, pH 7-8
Contains	0.09% sodium azide
Storage conditions	4° C

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

Target Information

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 2310032M22Rik; BAF180; BRG1-associated factor 180; hPB1; Polybromo-1D; Protein polybromo-1

Gene Aliases: 2610016F04Rik; AI507524; BAF180; PB1; PBRM1

UniProt ID: (Human) Q86U86

Entrez Gene ID: (Human) 55193, (Mouse) 66923

Molecular Function: chromatin/chromatin-binding, or -regulatory protein

chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated mitotic nuclear division negative regulation of cell proliferation covalent chromatin modification placenta development blood vessel remodeling heart morphogenesis epicardium-derived cardiac endothelial cell differentiation heart development chromatin modification positive regulation of transcription, DNA-templated cardiac vascular smooth muscle cell development vasculogenesis involved in coronary vascular morphogenesis

Process(es)

DNA binding chromatin binding protein binding