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Invitrogen
PBX3 Polyclonal Antibody
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FIGURE: 1 / 2
PBX3 Antibody (PA5-102989) in ICC/IF
Immunocytochemistry analysis of PBX3 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with PBX3 polyclonal antibody (Product # PA5-102989) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-102989
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PBX3(Accession P40426), corresponding to amino acid residues Q9-Q59.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total PBX3.
Target Information
PBX3 is a transcriptional activator that binds the sequence 5'-ATCAATCAA-3'.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Homeobox protein PBX3; pre-B-cell leukemia homeobox 3; Pre-B-cell leukemia transcription factor 3; unnamed protein product
Gene Aliases: PBX3
UniProt ID: (Human) P40426, (Mouse) O35317
Entrez Gene ID: (Human) 5090, (Mouse) 18516
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding
protein binding
homeodomain transcription factor
eye development
regulation of respiratory gaseous exchange by neurological system process
regulation of transcription, DNA-templated
anterior compartment pattern formation
posterior compartment specification
brain development
respiratory gaseous exchange
adult locomotory behavior
organ morphogenesis
dorsal spinal cord development
positive regulation of transcription from RNA polymerase II promoter
embryonic organ development
neuron development
transcription, DNA-templated
transcription from RNA polymerase II promoter
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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