Invitrogen

PCBD1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (5) PCBD1 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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PCBD1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Pterin-4-alpha-carbinolamine dehydratase was performed using 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PCBD1 Polyclonal Antibody (Product # PA5-58017) at 2 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing predominantly nuclear and cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

PCBD1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of PCBD1 in human rectal tissue shows strong cytoplasmic positivity in endocrine cells. Samples were probed using a PCBD1 Polyclonal Antibody (Product # PA5-58017).

PCBD1 Antibody in Western Blot (WB) — Western blot was performed using Anti-PCBD1 Polyclonal Antibody (Product # PA5-58017) and a 12 kDa band corresponding to Pterin-4-alpha-carbinolamine dehydratase was observed across cell lines and tissue extracts tested and not in MOLT-4, Reh, Mouse Spleen and Mouse Brain. Whole cell extracts (30 µg lysate) of Caco-2 (Lane 1), Hep G2 (Lane 2), A549 (Lane 3), U-2 OS (Lane 4), MOLT-4 (Lane 5), Reh (Lane 6) and tissue extracts of Mouse Spleen (Lane 7), Mouse Brain (Lane 8), Mouse Kidney (Lane 9), Mouse Liver (Lane 10) and Rat Liver (Lane 11) were electrophoresed using Novex™ 16% Tricine Protein Gel (Product # EC66952BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

PCBD1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines and tissues owing to their inherent genetic constitution. Relative expression of Pterin-4-alpha-carbinolamine dehydratase was observed in Caco-2, Hep G2, A549, U-2 OS, Mouse Kidney, Mouse Liver, Rat Liver in comparison to MOLT-4, Reh, Mouse Spleen and Mouse Brain using Anti-PCBD1 Polyclonal Antibody (Product # PA5-58017) in Western Blot. {RE}

Product Details

PA5-58017

Applications	Tested Dilution	Publications
Western Blot (WB)	1 µg/mL	-
Immunohistochemistry (IHC)	1:200-1:500	-
Immunocytochemistry (ICC/IF)	0.25-2 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human PCBD1 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2645241

Product Specific Information

Immunogen sequence: MAGKAHRLSA EERDQLLPNL RAVGWNELEG RDAIFKQFHF KDFNRAFGFM TR

Highest antigen sequence identity to the following orthologs: Mouse - 98%, Rat - 100%.

Target Information

PCBD1 encodes pterin-4 alpha-carbinolamine dehydratase, an enzyme involved in phenylalanine hydroxylation. A deficiency of this enzyme leads to hyperphenylalaninemia. The enzyme regulates the homodimerization of the transcription factor hepatocyte nuclear factor 1 (HNF1).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 4-alpha-hydroxy-tetrahydropterin dehydratase; 6-pyruvoyl-tetrahydropterin synthase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1); DCoH; Dimerization cofactor of hepatocyte nuclear factor 1-alpha; dimerization cofactor of HNF1; dimerizing cofactor for HNF1; PCD; Phenylalanine hydroxylase-stimulating protein; PHS; pt; Pterin carbinolamine dehydratase; pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha; Pterin-4-alpha-carbinolamine dehydratase

Gene Aliases: DCOH; PCBD; PCBD1; PCD; PHS; TCF1

UniProt ID: (Human) P61457, (Mouse) P61458, (Rat) P61459

Entrez Gene ID: (Human) 5092, (Mouse) 13180, (Rat) 29700

Molecular Function: dehydratase