Western blot analysis of PCCA in (lane 2) Gene specific transient over-expression HEK293T cell lysate and (lane 3) Empty vector transfected control HEK293T cell lysate. Samples were probed with a PCCA polyclonal antibody (Product # PA1-27572) at a dilution of 1:1000. Secondary detection was performed using a peroxidase conjugated Rabbit anti-Chicken IgY (IgG) at a 1:10,000 dilution and developed with TMB Substrate.
|Tested species reactivity||Human|
|Host / Isotype||Chicken / IgY|
|Immunogen||Synthetic peptide corresponding to human Propionyl-CoA carboxylase alpha chain. Mitochondrial.|
|Purification||Antigen affinity chromatography|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-27572 detects PCCA from human samples.
PA1-27572 may be successfully used in Western blot applications. By Western blot, this antibody has only been tested on fusion protein.
The immunogen is a synthetic peptide corresponding to human Propionyl-CoA carboxylase alpha chain. Mitochondrial.
PCCA encodes the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency result in
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.