Invitrogen

PCCB Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (11) PCCB antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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PCCB Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of PCCB in human skin using PCCB Polyclonal Antibody (Product # PA5-57880) shows only weak cytoplasmic positivity in a subset of epidermal cells.

PCCB Antibody in Western Blot (WB) — Western blot analysis of PCCB using PCCB Polyclonal Antibody (Product # PA5-57880) (A) shows similar pattern to an independent PCCB Polyclonal Antibody (B).

PCCB Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of PCCB demonstrates antibody specificity. Immunohistochemical analysis of PCCB using anti-PCCB Polyclonal Antibody (Product # PA5-57880), shows significant staining of PCCB in human liver and shows minimal or weak staining in human skin tissues. The relative expression levels of PCCB within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-57880

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (IHC)	1:1,000-1:2,500	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human PCCB View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2645247

Product Specific Information

Immunogen sequence: SSKHLCGDTN YAWPTAEIAV MGAKGAVEII FKGHENVEAA QAEYIEKFAN PFPAAVRGFV DDIIQPSSTR ARICCDLDVL

Highest antigen sequence identity to the following orthologs: Mouse - 94%, Rat - 94%.

Target Information

The protein encoded by this gene is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits. This gene encodes the beta subunit of PCC. Defects in this gene are a cause of propionic acidemia type II (PA-2).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: PCCase subunit beta; Propanoyl-CoA:carbon dioxide ligase subunit beta; propionyl CoA carboxylase, beta polypeptide; propionyl Coenzyme A carboxylase, beta polypeptide; Propionyl-CoA carboxylase beta chain, mitochondrial

Gene Aliases: PCCB

UniProt ID: (Human) P05166

Entrez Gene ID: (Human) 5096

Molecular Function: ligase metabolite interconversion enzyme