|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the C-terminal region of human PCDGF|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Granulin, a novel STAT3-interacting protein, enhances STAT3 transcriptional function and correlates with poorer prognosis in breast cancer.
40-3400 was used in immunocytochemistry, immunoprecipitation, and western blot to identify novel STAT3-interacting proteins in breast cancer cells
|Yeh JE,Kreimer S,Walker SR,Emori MM,Krystal H,Richardson A,Ivanov AR,Frank DA||Genes and cancer (6:153)||2015|
Evidence of the innate antiviral and neuroprotective properties of progranulin.
40-3400 was used in western blot to examine the role of progranulin during HIV infection of the central nervous system.
|Suh HS,Lo Y,Choi N,Letendre S,Lee SC||PloS one (9:null)||2014|
Progranulin does not bind tumor necrosis factor (TNF) receptors and is not a direct regulator of TNF-dependent signaling or bioactivity in immune or neuronal cells.
40-3400 was used in western blot to test if aberrant PGRN-TNFR interactions underlie the molecular basis for neuroinflammation.
|Chen X,Chang J,Deng Q,Xu J,Nguyen TA,Martens LH,Cenik B,Taylor G,Hudson KF,Chung J,Yu K,Yu P,Herz J,Farese RV,Kukar T,Tansey MG||The Journal of neuroscience : the official journal of the Society for Neuroscience (33:9202)||2013|
Proteolytic processing of TAR DNA binding protein-43 by caspases produces C-terminal fragments with disease defining properties independent of progranulin.
40-3400 was used in western blot to examine proteolysis of TAR DNA binding protein-43.
|Dormann D,Capell A,Carlson AM,Shankaran SS,Rodde R,Neumann M,Kremmer E,Matsuwaki T,Yamanouchi K,Nishihara M,Haass C||Journal of neurochemistry (110:1082)||2009|
|Human||1:100||Missense mutations in the progranulin gene linked to frontotemporal lobar degeneration with ubiquitin-immunoreactive inclusions reduce progranulin production and secretion.||Shankaran SS,Capell A,Hruscha AT,Fellerer K,Neumann M,Schmid B,Haass C||The Journal of biological chemistry (283:1744)||2008|
||Missense mutations in the progranulin gene linked to frontotemporal lobar degeneration with ubiquitin-immunoreactive inclusions reduce progranulin production and secretion.||Shankaran SS,Capell A,Hruscha AT,Fellerer K,Neumann M,Schmid B,Haass C||The Journal of biological chemistry (283:1744)||2008|