Western blot of PCDH17 in mouse fetal brain lysate was performed using 35ug protein in RIPA buffer. Lysates were probed with a PCDH17 polyclonal antibody (Product# PA5-37941) at a dilution of 0.2ug/ml and incubated for 1 hour, followed by chemiluminescent detection.
|Tested species reactivity||Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-RVFADVHSRASRDS, from the internal region near the C-terminus of PCDH17 (aa1098-111)|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-0.3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with human, rat, pig and cow based on sequence homology.
This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. The encoded protein contains six extracellular cadherin domains, a transmembrane domain, and a cytoplasmic tail differing from those of the classical cadherins. The encoded protein may play a role in the establishment and function of specific cell-cell connections in the brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.