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Western blot analysis of PCF11 using A) 30 µg 293T whole cell extract (B) 30 µg A431 whole cell extract and C) 30 µg HeLa whole cell extract. Samples were loaded onto a 5% SDS-PAGE gel and probed with a PCF11 polyclonal antibody (Product # PA5-35943) at a dilution of 1:500.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1309 and 1524 of PCF11|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Component of pre-mRNA cleavage complex II.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
cleavage and polyadenylation factor subunit; homolog S. cerevisiae; PCF11; PCF11, cleavage and polyadenylation factor II subunit, homolog; PCF11, cleavage and polyadenylation factor subunit, homolog; PCF11p homolog; pre-mRNA cleavage complex II protein Pcf11