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Invitrogen
PCK1 Polyclonal Antibody
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FIGURE: 1 / 2
PCK1 Antibody (720266) in WB
Western blot was performed using Anti-PCK1 Polyclonal Antibody (Product # 720266) and a 34 kDa band corresponding to PCK1 was observed across the panel tested. Whole cell extracts (30 µg lysate) of MCF-7 (Lane 1), MDA-MB-231 (Lane 2), Hep G2 (Lane 3), HEK-293 (Lane 4), Caco-2 (Lane 5), HCT 116 (Lane 6) and Mouse Liver (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product... View More
Product Details
720266
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Protein corresponding to Human PCK1 (aa 472-620)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 30% glycerol, 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.
This antibody is predicted to react with Monkey, Mouse, Sheep and Bovine
Target Information
Phosphoenolpyruvate Carboxykinase 1 (PCK1) is a main control point for the regulation of gluconeogenesis. During gluconeogenesis PCK1 acts as the rate-limiting enzyme. PCK1 regulates the formation and maintenance of memory CH8(+) T-cells via gluconeogenesis. PCK1 aids in controlling the levels of metabolic intermediates in the citric acid cycle by regulating cataplerosis and anaplerosis. When glucose levels are high, PCK1 catalyzes the cataplerotic conversion of oxaloacetate to phosphoenolpyruvate (PEP). When they are low it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate. When phosphorylated at Ser-90 by AKT1, PCK1 acts as a protein kinase by reducing the binding affinity to oxaloacetate and promotes an atypical serine protein kinase activity using GTP as the donor. This activity regulates lipogenesis by disrupting the interaction between INSIG proteins and SCAP and promoting nuclear translocation of SREBP proteins and transcription of lipogenesis-related genes. An important paralog of this gene is PCK2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HGNC:8724; MGC22652; PE PEPCKC; PEP carboxykinase; PEPCK-C; phosphoenolpyruvate carboxykinase (GTP); phosphoenolpyruvate carboxykinase 1 (soluble); phosphoenolpyruvate carboxykinase 1, cytosolic; phosphoenolpyruvate carboxykinase, cytosolic; phosphoenolpyruvate carboxykinase, cytosolic (GTP); Phosphoenolpyruvate carboxykinase, cytosolic [GTP]; phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase; Serine-protein kinase PCK1; unnamed protein product
Gene Aliases: AI265463; GTP; PCK; Pck-1; PCK1; PCKDC; PEPCK; PEPCK-C; PEPCK1; PEPCKC; RATPEPCK
UniProt ID: (Human) P35558, (Rat) P07379, (Mouse) Q9Z2V4
Entrez Gene ID: (Human) 5105, (Rat) 362282, (Mouse) 18534
nucleotide binding
magnesium ion binding
phosphoenolpyruvate carboxykinase activity
phosphoenolpyruvate carboxykinase (GTP) activity
GTP binding
kinase activity
transferase activity
lyase activity
carboxy-lyase activity
purine nucleotide binding
manganese ion binding
carboxylic acid binding
metal ion binding
protein serine kinase activity (using GTP as donor)
GDP binding
kinase
glucose metabolic process
gluconeogenesis
oxaloacetate metabolic process
NLS-bearing protein import into nucleus
lipid metabolic process
response to bacterium
peptidyl-serine phosphorylation
propionate catabolic process
response to insulin
cellular response to insulin stimulus
SREBP-SCAP complex retention in endoplasmic reticulum
glucose homeostasis
response to starvation
positive regulation of memory T cell differentiation
dicarboxylic acid metabolic process
positive regulation of cholesterol biosynthetic process
positive regulation of transcription from RNA polymerase II promoter
glyceraldehyde-3-phosphate biosynthetic process
glycerol biosynthetic process from pyruvate
positive regulation of lipid biosynthetic process
regulation of lipid biosynthetic process
cellular response to potassium ion starvation
protein maturation
hepatocyte differentiation
cellular response to glucose stimulus
cellular response to dexamethasone stimulus
tricarboxylic acid metabolic process
internal protein amino acid acetylation
response to activity
positive regulation of transcription from RNA polymerase II promoter in response to acidic pH
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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