Search
Invitrogen
PCK1 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 2
PCK1 Antibody (720266) in WB
Western blot was performed using Anti-PCK1 Polyclonal Antibody (Product # 720266) and a 34 kDa band corresponding to PCK1 was observed across the panel tested. Whole cell extracts (30 µg lysate) of MCF-7 (Lane 1), MDA-MB-231 (Lane 2), Hep G2 (Lane 3), HEK-293 (Lane 4), Caco-2 (Lane 5), HCT 116 (Lane 6) and Mouse Liver (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
720266
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Protein corresponding to Human PCK1 (aa 472-620)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 30% glycerol, 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.
This antibody is predicted to react with Monkey, Mouse, Sheep and Bovine
Target Information
Phosphoenolpyruvate Carboxykinase 1 (PCK1) is a main control point for the regulation of gluconeogenesis. During gluconeogenesis PCK1 acts as the rate-limiting enzyme. PCK1 regulates the formation and maintenance of memory CH8(+) T-cells via gluconeogenesis. PCK1 aids in controlling the levels of metabolic intermediates in the citric acid cycle by regulating cataplerosis and anaplerosis. When glucose levels are high, PCK1 catalyzes the cataplerotic conversion of oxaloacetate to phosphoenolpyruvate (PEP). When they are low it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate. When phosphorylated at Ser-90 by AKT1, PCK1 acts as a protein kinase by reducing the binding affinity to oxaloacetate and promotes an atypical serine protein kinase activity using GTP as the donor. This activity regulates lipogenesis by disrupting the interaction between INSIG proteins and SCAP and promoting nuclear translocation of SREBP proteins and transcription of lipogenesis-related genes. An important paralog of this gene is PCK2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HGNC:8724; MGC22652; PE PEPCKC; PEP carboxykinase; phosphoenolpyruvate carboxykinase (GTP); phosphoenolpyruvate carboxykinase 1 (soluble); phosphoenolpyruvate carboxykinase 1, cytosolic; phosphoenolpyruvate carboxykinase, cytosolic; phosphoenolpyruvate carboxykinase, cytosolic (GTP); phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase; serine-protein kinase PCK1; unnamed protein product
Gene Aliases: GTP; PCK; Pck-1; PCKDC; PEPCK; PEPCK-C; PEPCK1; PEPCKC; RATPEPCK
UniProt ID: (Human) P35558, (Mouse) Q9Z2V4, (Rat) P07379
Entrez Gene ID: (Human) 5105, (Mouse) 18534, (Rat) 362282
magnesium ion binding
phosphoenolpyruvate carboxykinase activity
phosphoenolpyruvate carboxykinase (GTP) activity
GTP binding
purine nucleotide binding
manganese ion binding
carboxylic acid binding
protein serine kinase activity (using GTP as donor)
nucleoside diphosphate kinase activity
GDP binding
glucose metabolic process
gluconeogenesis
oxaloacetate metabolic process
glycerol biosynthetic process
NLS-bearing protein import into nucleus
lipid metabolic process
response to bacterium
response to activity
peptidyl-serine phosphorylation
response to nutrient levels
response to lipopolysaccharide
response to insulin
cellular response to insulin stimulus
response to lipid
SREBP-SCAP complex retention in endoplasmic reticulum
glucose homeostasis
response to starvation
positive regulation of memory T cell differentiation
dicarboxylic acid metabolic process
positive regulation of cholesterol biosynthetic process
positive regulation of transcription from RNA polymerase II promoter
glyceraldehyde-3-phosphate biosynthetic process
positive regulation of lipid biosynthetic process
regulation of lipid biosynthetic process
cellular response to potassium ion starvation
protein maturation
hepatocyte differentiation
response to interleukin-6
cellular response to retinoic acid
cellular response to cAMP
cellular response to fructose stimulus
cellular response to glucose stimulus
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular response to glucagon stimulus
cellular response to hypoxia
cellular hyperosmotic response
cellular hyperosmotic salinity response
cellular hypotonic response
cellular hypotonic salinity response
cellular response to dexamethasone stimulus
tricarboxylic acid metabolic process
cellular response to raffinose
cellular response to phorbol 13-acetate 12-myristate
response to methionine
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support