Immunofluorescent analysis of PCK2 showing staining in the mitochondria of Hep G2 cells. Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min and stained using a PCK2 polyclonal antibody (Product # PA5-28078) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 357 and 449 of Human PCK2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 40% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-28078 targets PCK2 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-28078 immunogen is synthetic peptide corresponding to a region within amino acids 357 and 449 of Human PCK2.
This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.