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Invitrogen
PCK2 Polyclonal Antibody
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FIGURE: 1 / 8
PCK2 Antibody (PA5-28078) in ICC/IF
PCK2 Polyclonal Antibody detects PCK2 protein at mitochondria by immunofluorescent analysis. Sample: Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: PCK2 protein stained by PCK2 Polyclonal Antibody (Product # PA5-28078) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-28078
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human PCK2. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 40% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: HepG2, Neuro2A, GL261, PC-12.
Predicted reactivity: Mouse (100%), Rat (100%), Pig (100%), Rhesus Monkey (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein; HGNC:8725; mtPCK2; PCK2; PEP carboxykinase; PEPCK-M; phosphoenolpyruvate carboxykinase 2 (mitochondrial) isoform PCK2_1; phosphoenolpyruvate carboxykinase 2 (mitochondrial) isoform PCK2_2; phosphoenolpyruvate carboxykinase 2 (mitochondrial) isoform PCK2_3; Phosphoenolpyruvate carboxykinase 2, mitochondrial; Phosphoenolpyruvate carboxykinase [GTP], mitochondrial; phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase; Serine/threonine-protein kinase PCK2; unnamed protein product
Gene Aliases: 1810010O14Rik; 9130022B02Rik; mtPCK2; PCK2; PEPCK; PEPCK-M; PEPCK2
UniProt ID: (Human) Q16822, (Mouse) Q8BH04
Entrez Gene ID: (Human) 5106, (Mouse) 74551, (Rat) 361042
gluconeogenesis
oxaloacetate metabolic process
propionate catabolic process
positive regulation of insulin secretion
response to lipopolysaccharide
cellular response to insulin stimulus
response to starvation
glycerol biosynthetic process from pyruvate
hepatocyte differentiation
cellular response to glucose stimulus
cellular response to tumor necrosis factor
response to dexamethasone
cellular response to dexamethasone stimulus
pyruvate metabolic process
NADH oxidation
response to glucocorticoid
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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