Proteintech

PCM1 Polyclonal Antibody

PCM1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

PCM1 Antibody (19856-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°CMethanol) fixed NIH/3T3 cells using 19856-1-AP (PCM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PCM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PCM1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

19856-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 8 publications 8 publications

Flow Cytometry (Flow)

Assay-dependent

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PCM1 Fusion Protein Ag13929 (1-254 aa encoded by BC000453)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MATGGGPFE DGMNDQDLPN WSNENVDDRL NNMDWGAQQK KANRSSEKNK KKFGVESDKR VTNDISPESS PGVGRRRTKT PHTFPHSRYM SQMSVPEQAE LEKLKQRINF SDLDQRSIGS DSQGRATAAN NKRQLSENRK PFNFLPMQIN TNKSKDASTS PPNRETIGSA QCKELFASAL SNDLLQNCQV SEEDGRGEPA MESSQIVSRL VQIRDYITKA SSMREDLVEK NERSANVERL THLIDHLKEQ EKSYM (1-254 aa encoded by BC000453)

Target Information

PCM-1 is required for centrosome assembly and function. This protein is essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. The protein is required to anchor microtubules to the centrosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Jmjd4 Facilitates Pkm2 Degradation in Cardiomyocytes and Is Protective Against Dilated Cardiomyopathy.

Circulation

Tang Y,Feng M,Su Y,Ma T,Zhang H,Wu H,Wang X,Shi S,Zhang Y,Xu Y,Hu S,Wei K,Xu D

Tue May 30 00:00:00 UTC 2023

TTBK2 controls cilium stability by regulating distinct modules of centrosomal proteins.

Molecular biology of the cell

Nguyen A,Goetz SC

Sun Jan 01 00:00:00 UTC 2023

Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis.

eLife

Hall EA,Kumar D,Prosser SL,Yeyati PL,Herranz-Pérez V,García-Verdugo JM,Rose L,McKie L,Dodd DO,Tennant PA,Megaw R,Murphy LC,Ferreira MF,Grimes G,Williams L,Quidwai T,Pelletier L,Reiter JF,Mill P

Wed Feb 15 00:00:00 UTC 2023

Actin filaments form a size-dependent diffusion barrier around centrosomes.

EMBO reports

Cheng H,Kao YL,Chen T,Sharma L,Yang WT,Chuang YC,Huang SH,Lin HR,Huang YS,Kao CL,Yang LW,Bearon R,Cheng HC,Hsia KC,Lin YC

Mon Jan 09 00:00:00 UTC 2023

The mechanism of acentrosomal spindle assembly in human oocytes.

Science (New York, N.Y.)

Wu T,Dong J,Fu J,Kuang Y,Chen B,Gu H,Luo Y,Gu R,Zhang M,Li W,Dong X,Sun X,Sang Q,Wang L

Fri Nov 18 00:00:00 UTC 2022

Diastolic dysfunction in a pre-clinical model of diabetes is associated with changes in the cardiac non-myocyte cellular composition.

Cardiovascular diabetology

Cohen CD,De Blasio MJ,Lee MKS,Farrugia GE,Prakoso D,Krstevski C,Deo M,Donner DG,Kiriazis H,Flynn MC,Gaynor TL,Murphy AJ,Drummond GR,Pinto AR,Ritchie RH

Tue Jun 01 00:00:00 UTC 2021

Novel loss-of-function mutation in HERC2 is associated with severe developmental delay and paediatric lethality.

Journal of medical genetics

Elpidorou M,Best S,Poulter JA,Hartill V,Hobson E,Sheridan E,Johnson CA

Sat May 01 00:00:00 UTC 2021

A WDR35-dependent coat protein complex transports ciliary membrane cargo vesicles to cilia.

eLife

Quidwai T,Wang J,Hall EA,Petriman NA,Leng W,Kiesel P,Wells JN,Murphy LC,Keighren MA,Marsh JA,Lorentzen E,Pigino G,Mill P

Thu Nov 04 00:00:00 UTC 2021

A ciliopathy complex builds distal appendages to initiate ciliogenesis.

The Journal of cell biology

Kumar D,Rains A,Herranz-Pérez V,Lu Q,Shi X,Swaney DL,Stevenson E,Krogan NJ,Huang B,Westlake C,Garcia-Verdugo JM,Yoder BK,Reiter JF

Mon Sep 06 00:00:00 UTC 2021

Aurora Kinase A proximity map reveals centriolar satellites as regulators of its ciliary function.

EMBO reports

Arslanhan MD,Rauniyar N,Yates JR,Firat-Karalar EN

Wed Aug 04 00:00:00 UTC 2021

Distinct Roles of TRAPPC8 and TRAPPC12 in Ciliogenesis via Their Interactions With OFD1.

Frontiers in cell and developmental biology

Zhang C,Li C,Siu GKY,Luo X,Yu S

Mon Sep 28 00:00:00 UTC 2020

Distinct Roles of TRAPPC8 and TRAPPC12 in Ciliogenesis via Their Interactions With OFD1.

Frontiers in cell and developmental biology

Zhang C,Li C,Siu GKY,Luo X,Yu S

Mon Sep 28 00:00:00 UTC 2020

CCDC57 Cooperates with Microtubules and Microcephaly Protein CEP63 and Regulates Centriole Duplication and Mitotic Progression.

Cell reports

Gurkaslar HK,Culfa E,Arslanhan MD,Lince-Faria M,Firat-Karalar EN

Tue May 12 00:00:00 UTC 2020

ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.

Developmental cell

Ran J,Liu M,Feng J,Li H,Ma H,Song T,Cao Y,Zhou P,Wu Y,Yang Y,Yang Y,Yu F,Guo H,Zhang L,Xie S,Li D,Gao J,Zhang X,Zhu X,Zhou J

Mon May 04 00:00:00 UTC 2020

Centriolar satellites are required for efficient ciliogenesis and ciliary content regulation.

EMBO reports

Odabasi E,Gul S,Kavakli IH,Firat-Karalar EN

Sat Jun 01 00:00:00 UTC 2019

Centriolar satellites are required for efficient ciliogenesis and ciliary content regulation.

EMBO reports

Odabasi E,Gul S,Kavakli IH,Firat-Karalar EN

Sat Jun 01 00:00:00 UTC 2019

Centrosomal and ciliary targeting of CCDC66 requires cooperative action of centriolar satellites, microtubules and molecular motors.

Scientific reports

Conkar D,Bayraktar H,Firat-Karalar EN

Thu Oct 03 00:00:00 UTC 2019

The centriolar satellite protein CCDC66 interacts with CEP290 and functions in cilium formation and trafficking.

Journal of cell science

Conkar D,Culfa E,Odabasi E,Rauniyar N,Yates JR,Firat-Karalar EN

Sat Apr 15 00:00:00 UTC 2017

The centriolar satellite protein CCDC66 interacts with CEP290 and functions in cilium formation and trafficking.

Journal of cell science

Conkar D,Culfa E,Odabasi E,Rauniyar N,Yates JR,Firat-Karalar EN

Sat Apr 15 00:00:00 UTC 2017

The X-linked deubiquitinase USP9X is an integral component of centrosome.

The Journal of biological chemistry

Wang Q,Tang Y,Xu Y,Xu S,Jiang Y,Dong Q,Zhou Y,Ge W

Fri Aug 04 00:00:00 UTC 2017

The X-linked deubiquitinase USP9X is an integral component of centrosome.

The Journal of biological chemistry

Wang Q,Tang Y,Xu Y,Xu S,Jiang Y,Dong Q,Zhou Y,Ge W

Fri Aug 04 00:00:00 UTC 2017

The X-linked deubiquitinase USP9X is an integral component of centrosome.

The Journal of biological chemistry

Wang Q,Tang Y,Xu Y,Xu S,Jiang Y,Dong Q,Zhou Y,Ge W

Fri Aug 04 00:00:00 UTC 2017

The X-linked deubiquitinase USP9X is an integral component of centrosome.

The Journal of biological chemistry

Wang Q,Tang Y,Xu Y,Xu S,Jiang Y,Dong Q,Zhou Y,Ge W

Fri Aug 04 00:00:00 UTC 2017

USP9X regulates centrosome duplication and promotes breast carcinogenesis.

Nature communications

Li X,Song N,Liu L,Liu X,Ding X,Song X,Yang S,Shan L,Zhou X,Su D,Wang Y,Zhang Q,Cao C,Ma S,Yu N,Yang F,Wang Y,Yao Z,Shang Y,Shi L

Fri Mar 31 00:00:00 UTC 2017

USP9X regulates centrosome duplication and promotes breast carcinogenesis.

Nature communications

Li X,Song N,Liu L,Liu X,Ding X,Song X,Yang S,Shan L,Zhou X,Su D,Wang Y,Zhang Q,Cao C,Ma S,Yu N,Yang F,Wang Y,Yao Z,Shang Y,Shi L

Fri Mar 31 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: hPCM-1; mPCM-1; PCM-1; Pericentriolar material 1 protein; pericentriolar material 1, PCM1; pericentriolar material gene 1

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Gene Aliases: 2600002H09Rik; 9430077F19Rik; C030044G17Rik; PCM1; PTC4; RET/PCM-1

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UniProt ID: (Human) Q15154, (Mouse) Q9R0L6

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Entrez Gene ID: (Human) 5108, (Mouse) 18536

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Function(s)

protein binding identical protein binding

Process(es)

G2/M transition of mitotic cell cycle neuron migration interkinetic nuclear migration cytoplasmic microtubule organization microtubule anchoring microtubule anchoring at centrosome nonmotile primary cilium assembly intraciliary transport involved in cilium morphogenesis cilium assembly negative regulation of neurogenesis centrosome organization protein localization to centrosome positive regulation of intracellular protein transport neuronal stem cell population maintenance cell projection organization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PCM1 Polyclonal Antibody

PCM1 Antibody (19856-1-AP) in ICC/IF

Product Details

19856-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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