Rockland

PCNA Polyclonal Antibody

Name: PCNA Polyclonal Antibody
Brand: Rockland
SKU: 600-401-968

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Additional Information:

Antibody Testing Data (2)

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PCNA Antibody (600-401-968) in WB

Western blot using Rocklands affinity purified anti-PCNA antibody shows detection of PCNA protein in HEK293 (lane 1) and Jurkat (lane 2) whole cell extracts. Approximately 25 µg of lysate was loaded per lane onto a 4-20% gradient gel followed by transfer to nitrocellulose. After blocking, the membrane was incubated with the primary antibody diluted to 1:1000. The membrane was washed and reacted with a 1:10,000 dilution of IRDye® 800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10 (611-132-122) (800 nm channel, green). Molecula... View More

Product Image Gallery

PAGE-MAP (microsphere affinity proteomics) of Rabbit Anti-PCNA Antibody (Catalog Number: 600-401-968, Lot Number: 32579). Antibody array western blot binding of gelfree size separated fractions of multiple lysates (solid lines) and shotgun mass spectroscopy identification (dashed lines) of the target band run in parallel correlate confirming the specificity of this antibody against PCNA. Data was provided by the Lund-Johansen lab of Oslo University Hospital. For more information on PAGE-MAP/IP-MS identification of antibody specificity and its large-scale implementation for antibody validation see Sikorski et. al., (2018) Nature Methods 15, 909-912.

Product Details

600-401-968

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:2,000

ELISA (ELISA)

1:2,000-1:8,000

Immunoprecipitation (IP)

1:500-1:2,000

Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Fish, Human, Mouse, Non-human primate, Rat, Xenopus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human PCNA protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.97 mg/mL

100 µg

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Directed against human PCNA protein. A BLAST analysis was used to suggest cross reactivity with PCNA protein from human, monkey, dog, mouse, rat, bovine, chicken, fish and Xenopus based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Target Information

PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cyclin; DNA polymerase delta auxiliary protein; DNA sliding clamp PCNA; HGCN8729; MGC8367; PCNA; Proliferating cell nuclear antigen; unnamed protein product

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Gene Aliases: ATLD2; PCNA; Pcna/cyclin; PCNAR

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UniProt ID: (Bovine) Q3ZBW4, (Human) P12004, (Chicken) Q9DEA3, (Rat) P04961, (Mouse) P17918

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Entrez Gene ID: (Bovine) 515499, (Dog) 477166, (Human) 5111, (Chicken) 373984, (Rat) 25737, (Mouse) 18538

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Function(s)

purine-specific mismatch base pair DNA N-glycosylase activity chromatin binding damaged DNA binding protein C-terminus binding receptor tyrosine kinase binding dinucleotide insertion or deletion binding MutLalpha complex binding histone acetyltransferase binding identical protein binding DNA polymerase binding DNA binding protein binding enzyme binding estrogen receptor binding DNA polymerase processivity factor activity macromolecular complex binding transcription factor binding DNA polymerase processivity factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter base-excision repair, gap-filling translesion synthesis epithelial cell differentiation replication fork processing positive regulation of deoxyribonuclease activity cellular response to UV positive regulation of DNA repair positive regulation of DNA replication cellular response to xenobiotic stimulus positive regulation of DNA-directed DNA polymerase activity mitotic telomere maintenance via semi-conservative replication DNA replication leading strand elongation regulation of DNA replication DNA repair mismatch repair chromatin organization cellular response to DNA damage stimulus response to oxidative stress heart development response to estradiol response to lipid estrous cycle response to cadmium ion cellular response to hydrogen peroxide response to dexamethasone liver regeneration response to L-glutamate regulation of catalytic activity

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PCNA Polyclonal Antibody

PCNA Antibody (600-401-968) in WB

Product Details

600-401-968

Western Blot (WB)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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