Immunofluorescent analysis of HeLa cells using a PCNA polyclonal antibody (Product # PA5-12449) at a dilution of 1:10-50. Primary antibody was detected with goat anti-rabbit lgG, fluor-conjugated secondary antibody (green). Actin filaments have been labeled with red dye conjugated phalloidin.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 89-117 amino acids from the central region of human PCNA|
|Purification||Size-exclusion - Dialysis, Ammonium sulfate precipitation|
|Contains||0.09% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:10-1:50|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, chicken, hamster, mouse, non-human primate and rat based on sequence homology.
PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.