Immunofluorescent analysis of PCNA in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min, using a PCNA polyclonal antibody (Product # PA5-27214). Green: Primary antibody at a dilution of 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 µm.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 253 of Human PCNA|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Contains||0.025% ProClin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Immunoprecipitation (IP)||5 µg|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Paraffin) (IHC (P))||See 2 publications below|
PA5-27214 targets PCNA in IF, IHC (P), and WB applications and shows reactivity with Human, mouse, and Rat samples.
The PA5-27214 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 253 of Human PCNA.
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Geraniol attenuates 2-acetylaminofluorene induced oxidative stress, inflammation and apoptosis in the liver of wistar rats.
PA5-27214 was used in immunohistochemistry - paraffin section to analyze attenuation of 2-acetylaminofluorene induced by oxidative stress, apoptosis and inflammation in the liver of wistar rats by geraniol
|Hasan SK,Sultana S||Toxicology mechanisms and methods (25:559)||2015|
|Not Applicable||Not Cited||
Therapeutic Touch Has Significant Effects on Mouse Breast Cancer Metastasis and Immune Responses but Not Primary Tumor Size.
PA5-27214 was used in immunohistochemistry - paraffin section to study the positive effects of therapeutic touch on mouse breast cancer metastasis and immune response, but not in primary tumor size
|Gronowicz G,Secor ER,Flynn JR,Jellison ER,Kuhn LT||Evidence-based complementary and alternative medicine : eCAM (2015:null)||2015|