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Immunofluorescent analysis of PCNA in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min, using a PCNA polyclonal antibody (Product # PA5-27214). Green: Primary antibody at a dilution of 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 µm.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 253 of Human PCNA|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Contains||0.025% ProClin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Immunoprecipitation (IP)||5 µg|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27214 targets PCNA in IF, IHC (P), and WB applications and shows reactivity with Human, mouse, and Rat samples.
The PA5-27214 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 253 of Human PCNA.
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
cyclin; DNA polymerase delta auxiliary protein; proliferating cell nuclear antigen
ATLD2; PCNA; Pcna/cyclin; PCNAR