|Tested species reactivity||Human|
|Published species reactivity||Rat, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant human PCNA protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:25|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control is tonsil, lymph node or small intestine tissue.
Expression of proliferating cell nuclear antigen (PCNA) or cyclin or polymerase delta auxiliary protein is elevated in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. PCNA/cyclin may act as an auxiliary protein of DNA polymerase-delta to play a fundamental role in the initiation of cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Flavonoids from each of the six structural groups reactivate BRM, a possible cofactor for the anticancer effects of flavonoids.
PA5-32541 was used in immunohistochemistry to study the ability of flavonoids to reactivate BRM and the significance for the anti-cancer effects of these compounds
|Kahali B,Marquez SB,Thompson KW,Yu J,Gramling SJ,Lu L,Aponick A,Reisman D||Carcinogenesis (35:2183)||2014|
Two drugs with paradoxical effects on liver regeneration through antiangiogenesis and antifibrosis: Losartan and Spironolactone: a pharmacologic dilemma on hepatocyte proliferation.
PA5-32541 was used in immunohistochemistry to study the effects of Losartan and Spironolactone on liver regeneration and the mechanisms involved
|Parlakgumus A,Colakoglu T,Kayaselcuk F,Colakoglu S,Ezer A,Cal¿skan K,Karakaya J,Yildirim S||The Journal of surgical research (179:60)||2013|
The protective effects of Ginkgo biloba extract (EGb-761) on radiation-induced dermatitis: an experimental study.
PA5-32541 was used in immunohistochemistry to study the protective effects of Ginkgo biloba extract on radiation-induced dermatitis
|Yirmibesoglu E,Karahacioglu E,Kilic D,Lortlar N,Akbulut G,Omeroglu S||Clinical and experimental dermatology (37:387)||2012|
Metastatic pancreatic cancer is dependent on oncogenic Kras in mice.
PA5-32541 was used in western blot to characterize a new mouse model of metastatic pancreatic adenocarcinoma
|Collins MA,Brisset JC,Zhang Y,Bednar F,Pierre J,Heist KA,Galbán CJ,Galbán S,di Magliano MP||PloS one (7:null)||2012|
Transgenic expression of interferon-¿ in mouse stomach leads to inflammation, metaplasia, and dysplasia.
PA5-32541 was used in western blot to study the induction of inflammation, metaplasia and displasia following transgenic expression of IFN-gamma in the murine stomach
|Syu LJ,El-Zaatari M,Eaton KA,Liu Z,Tetarbe M,Keeley TM,Pero J,Ferris J,Wilbert D,Kaatz A,Zheng X,Qiao X,Grachtchouk M,Gumucio DL,Merchant JL,Samuelson LC,Dlugosz AA||The American journal of pathology (181:2114)||2012|
|Not Applicable||Not Cited||
Essential role of ribosomal protein L11 in mediating growth inhibition-induced p53 activation.
PA5-32541 was used in western blot to study the role of the L11 ribosomal protein in activating p53 in response to conditions that inhibit cell growth
|Bhat KP,Itahana K,Jin A,Zhang Y||The EMBO journal (23:2402)||2004|