This antibody detects human PD-L1 isoform 1 (290aa, 33.3 kDa) and isoform 3 (178aa, 20.5 kDa), as well as mouse and rat PD-L1 (290aa, 33 kDa). It will not cross-react with PD-L2. By Western blot, this antibody has been observed to detect a band at 37 kDa.
Recommended positive controls: HeLa cell lysate, Daudi cell lysate, K562 cell lysate, human heart tissue/lysate.
PA5-20343 can be used with blocking peptide PEP-0463.
The PA5-20343 immunogen is located within amino acids 60-110 of PD-L1.
Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B7 homolog 1; B7-H1; CD274; CD274 antigen; PD-L1; PDCD1 ligand 1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; sCD274; soluble CD274; soluble PD L1; sPD L1
Gene Aliases: A530045L16Rik; B7-H; B7H1; CD274; PD-L1; PDCD1L1; PDCD1LG1; PDL1; RGD1566211
Molecular Function: immunoglobulin receptor superfamily