Proteintech

PD-L1/CD274 Polyclonal Antibody

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PD-L1/CD274 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunofluorescent analysis of (4% PFA) fixed human tonsilitis tissue using PD-L1/CD274 (C-terminal) antibody (28076-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 CD3 antibody (CL594-60181, Clone: 3F3A1, red).

PD-L1/CD274 Antibody in Western Blot (WB) — Untreated and PNGase F-treated lysates of Human placenta were subjected to SDS PAGE followed by western blot with 28076-1-AP (PD-L1/CD274 (C-terminal) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

PD-L1/CD274 Antibody in Immunoprecipitation (IP) — IP result of anti-PD-L1/CD274 (C-terminal)(IP:28076-1-AP, 4 µg; Detection:28076-1-AP 1:500) with human placenta tissue lysate 800 µg.

Product Details

28076-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:600	-
Immunohistochemistry (Paraffin) (IHC (P))	1:800	-
Immunoprecipitation (IP)	1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PD-L1/CD274 Fusion Protein Ag27557
Conjugate	Unconjugated
Form	Liquid
Concentration	233 µg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: B7 homolog 1; B7-H1; CD274; CD274 antigen; PD-L1; PDCD1 ligand 1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; sCD274; soluble CD274; soluble PD L1; sPD L1

Gene Aliases: A530045L16Rik; B7-H; B7H1; CD274; PD-L1; PDCD1L1; PDCD1LG1; PDL1; RGD1566211

UniProt ID: (Human) Q9NZQ7, (Mouse) Q9EP73

Entrez Gene ID: (Human) 29126, (Mouse) 60533, (Rat) 499342

Molecular Function: immunoglobulin receptor superfamily