Proteintech

PDCD6 Polyclonal Antibody

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PDCD6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 12303-1-AP ( PDCD6 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

PDCD6 Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 12303-1-AP (PDCD6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PDCD6 Antibody in Immunoprecipitation (IP) — IP result of anti-PDCD6 (IP:12303-1-AP, 3ug; Detection:12303-1-AP 1:500) with HeLa cells lysate 3200ug.

Product Details

12303-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:4,000	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	-	View 2 publications 2 publications
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PDCD6 Fusion Protein Ag2949 (1-191 aa encoded by BC012384) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.37 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MAAYSYRPG PGAGPGPAAG AALPDQSFLW NVFQRVDKDR SGVISDTELQ QALSNGTWTP FNPVTVRSII SMFDRENKAG VNFSEFTGVW KYITDWQNVF RTYDRDNSGM IDKNELKQAL SGFGYRLSDQ FHDILIRKFD RQGRGQIAFD DFIQGCIVLQ RLTDIFRRYD TDQDGWIQVS YEQYLSMVFS IV (1-191 aa encoded by BC012384)

Target Information

An increased intracellular Ca2+ concentration induces apoptotic cell death. Transiently elevated Ca2+ concentrations are required for glucocorticoid-mediated and T cell receptor-mediated pathways, leading to T cell apoptosis. ALG-2 (for apoptosis-linked gene 2) is a Ca2+ -binding protein that participates in regulatory events occuring late in the apoptotic program and where several death signals converge. ALG-2 is a protein expressed in normal brain, and to a greater extent in ischemic brain. The ALG-2 protein contains five EF-hand-like motifs and shares homology with members of the penta EF-hand family, which includes Calpain small subunits sorcin and Grancalcin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Triggered recruitment of ESCRT machinery promotes endolysosomal repair.

Science (New York, N.Y.)

Skowyra ML,Schlesinger PH,Naismith TV,Hanson PI

Fri Apr 06 00:00:00 EDT 2018

Regulation of the CUL3 Ubiquitin Ligase by a Calcium-Dependent Co-adaptor.

Cell

McGourty CA,Akopian D,Walsh C,Gorur A,Werner A,Schekman R,Bautista D,Rape M

Thu Oct 06 00:00:00 EDT 2016

Regulation of the CUL3 Ubiquitin Ligase by a Calcium-Dependent Co-adaptor.

Cell

McGourty CA,Akopian D,Walsh C,Gorur A,Werner A,Schekman R,Bautista D,Rape M

Thu Oct 06 00:00:00 EDT 2016

Luteinizing hormone inhibits cisplatin-induced apoptosis in human epithelial ovarian cancer cells.

Oncology letters

Xia L,Wen H,Han X,Tang J,Huang Y

Tue Mar 01 00:00:00 EST 2016

FSH inhibits ovarian cancer cell apoptosis by up-regulating survivin and down-regulating PDCD6 and DR5.

Endocrine-related cancer

Huang Y,Jin H,Liu Y,Zhou J,Ding J,Cheng KW,Yu Y,Feng Y

Tue Feb 01 00:00:00 EST 2011

Bioinformatics

Protein Aliases: ALG-2; ALG-257; Apoptosis-linked gene 2 protein; Apoptosis-linked gene 2 protein homolog; PMP41; probable calcium-binding protein ALG-2; Programmed cell death protein 6

Gene Aliases: ALG-2; ALG2; AV299538; MA-3; PDCD6; PEF1B; PS2

UniProt ID: (Human) O75340, (Rat) G3V7W1, (Mouse) P12815

Entrez Gene ID: (Human) 10016, (Rat) 308061, (Mouse) 18570

Molecular Function: actin or actin-binding cytoskeletal protein