Western blot of PDE10A in various rat brain regions: (lane 1) Frontal Cortex (lane 2) Parietal Cortex (lane 3) Striatum (lane 4) Amygdela (lane 5) Hypothalamus (lane 6) Hippocampus (lane 7) Olfactory Bulb (lane 8) brain Stem (lane 9) Cerebellum (lane 10) Spinal Cord and (lane 11) whole brain using a PDE10A polyclonal antibody (Product # PA1-31145) at a dilution of 1:500.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (Human) against a common sequence near the 250-325 amino acids downstream from the N terminal region of PDE10A.|
|Purification||Antigen affinity chromatography|
|Storage buffer||proprietary buffer|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||Assay dependent|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-31145 detects PDE10A from mouse, rat, human samples.
PA1-31145 has been successfully used in immunohistochemistry, immunoprecipitation, Western blot applications.
The PA1-31145 immunogen is: Synthetic peptide (Human) against a common sequence near the 250-325 amino acids downstream from the N terminal region of PDE10A.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
Various cellular responses are regulated by the second messengers cAMP and cGMP. Phosphodiesterases, such as PDE10A, eliminate cAMP- and cGMP-mediated intracellular signaling by hydrolyzing the cyclic nucleotide to the corresponding nucleoside 5-prime monophosphate (Fujishige et al., 2000 [PubMed 10998054]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.