Invitrogen

PDE10A Polyclonal Antibody

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PDE10A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PDE10A (Product # PA5-31293) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

PDE10A Antibody in Western Blot (WB) — Western Blot analysis of PDE10A was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PDE10A Polyclonal Antibody (Product # PA5-31293) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

PDE10A Antibody in Immunohistochemistry (IHC) — Figure 3 Immunohistochemistry of renal cell carcinoma (patient 4) (A, B) Phosphodiesterase 10A (PDE10A) immunohistochemistry in normal control human kidney tissue. The high power image (B) indicates the collecting tubular epithelium with marked expression of PDE10A. The kidney carcinoma tissue of patient 4 shows foci of variable PDE10A immunoreactivity: moderate expression (C, D) or scant PDE10A expression (E, F) in different parts of the tumor. Scale bars in A, C, and E = 100 mum. Scale bars in B, D, and F = 20 mum.

Product Details

PA5-31293

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:20,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Not Applicable
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 547 and 779 of Human PDE10A View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1.97 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2548767

Product Specific Information

Recommended positive controls: HCT116, mouse brain, rat brain, Myc-DDK-tagged PDE10A-transfected 293T.

Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (84%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Various cellular responses are regulated by the second messengers cAMP and cGMP. Phosphodiesterases, such as PDE10A, eliminate cAMP- and cGMP-mediated intracellular signaling by hydrolyzing the cyclic nucleotide to the corresponding nucleoside 5-prime monophosphate.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Phosphodiesterase 10A IgG: A novel biomarker of paraneoplastic neurologic autoimmunity.

Neurology

Zekeridou A,Kryzer T,Guo Y,Hassan A,Lennon V,Lucchinetti CF,Pittock S,McKeon A

Published figure using PDE10A polyclonal antibody (Product # PA5-31293) in Immunohistochemistry

Tue Aug 20 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A; dJ416F21.1 (phosphodiesterase 10A); dual-substrate cAMP and cGMP, cyclic nucleotide phosphodiesterase; FLJ11894; FLJ25677; PDE10A2 brain-specific isoform; phosphodiesterase 10A1 (PDE10A1); phosphodiesterase 10A7 (PDE10A7); testis-specific phosphodiesterase PDE10A2

Gene Aliases: ADSD2; HSPDE10A; IOLOD; LINC00473; PDE10A; PDE10A19; Pde10a3

UniProt ID: (Human) Q9Y233, (Mouse) Q8CA95, (Rat) Q9QYJ6

Entrez Gene ID: (Human) 10846, (Mouse) 23984, (Rat) 63885

Molecular Function: phosphodiesterase