Invitrogen

PDE3A Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (12) PDE3A antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

PDE3A Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of PDE3A in HeLa cells using a PDE3A polyclonal antibody (Product # PA5-82503). The analysis shows localization to cytosol.

PDE3A Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of PDE3A in human colon using a PDE3A polyclonal antibody (Product # PA5-82503). The analysis shows strong cytoplasmic positivity in glandular cells.

PDE3A Antibody in Western Blot (WB) — Western blot analysis of PDE3A by a PDE3A polyclonal antibody (Product # PA5-82503). Analysis in human cell lines HeLa and HEK293.

PDE3A Antibody in Relative expression — Relative expression in western blot: Antibody specificity was demonstrated by detection of known differential basal expression of the target across tissue/cell models. Expression of PDE3A was observed specifically in HeLa cells and in HEK293 cells using anti-PDE3A Polyclonal Antibody (Product #PA5-82503) in western blot. The relative expression levels of PDE3A within each cell line is shown using RNA-Seq. {RE}

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

0.25-2 µg/mL

Immunofluorescence (IF)

0.25-2 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Western Blot (WB)

0.04-0.4 µg/mL

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human PDE3A

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2789661

Product Specific Information

Immunogen sequence: SPQILTPPVI CSSCGRPYSQ GNPADEPLER SGVATRTPSR TDDTAQVTSD YETNNNSDSS DIVQNEDETE CLREPLRKAS ACSTYAPETM MFLDKPILAP EPLVMDNLDS IMEQLNT

Target Information

Cyclic nucleotide phosphodiesterases (PDEs) comprise a complex group of enzymes, and at least 5 major PDE families or classes with distinctive properties have been identified. Members of the cGMP-inhibited cAMP PDE (cGI-PDE) family, such as PDE3A, are characterized by high affinity for cAMP and cGMP and competitive inhibition of their cAMP hydrolytic activity by cGMP and certain positive inotropic agents (Meacci et al., 1992 [PubMed 1315035]).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Cite this antibody

Bioinformatics

Protein Aliases: cAMP phosphodiesterase, myocardial cGMP-inhibited; CGI-PDE A; cGMP-inhibited 3',5'-cyclic phosphodiesterase A; Cyclic GMP-inhibited phosphodiesterase A; phosphodiesterase 3A, cGMP-inhibited

Gene Aliases: CGI-PDE; CGI-PDE A; CGI-PDE-A; HTNB; PDE3A

UniProt ID: (Human) Q14432

Entrez Gene ID: (Human) 5139

Molecular Function: hydrolase phosphodiesterase