Invitrogen

PDE3A Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (21) PDE3A antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

PDE3A Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of PDE3A in HeLa cells using a PDE3A polyclonal antibody (Product # PA5-82503). The analysis shows localization to cytosol.

PDE3A Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of PDE3A in human colon using a PDE3A polyclonal antibody (Product # PA5-82503). The analysis shows strong cytoplasmic positivity in glandular cells.

PDE3A Antibody in Western Blot (WB) — Western blot analysis of PDE3A by a PDE3A polyclonal antibody (Product # PA5-82503). Analysis in human cell lines HeLa and HEK293.

PDE3A Antibody — Relative expression in western blot: Antibody specificity was demonstrated by detection of known differential basal expression of the target across tissue/cell models. Expression of PDE3A was observed specifically in HeLa cells and in HEK293 cells using anti-PDE3A Polyclonal Antibody (Product #PA5-82503) in western blot. The relative expression levels of PDE3A within each cell line is shown using RNA-Seq. {RE}

Product Details

PA5-82503

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	1:200-1:500	-
Immunocytochemistry (ICC/IF)	0.25-2 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human PDE3A View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.1 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2789661

Product Specific Information

Immunogen sequence: SPQILTPPVI CSSCGRPYSQ GNPADEPLER SGVATRTPSR TDDTAQVTSD YETNNNSDSS DIVQNEDETE CLREPLRKAS ACSTYAPETM MFLDKPILAP EPLVMDNLDS IMEQLNT

Target Information

Cyclic nucleotide phosphodiesterases (PDEs) comprise a complex group of enzymes, and at least 5 major PDE families or classes with distinctive properties have been identified. Members of the cGMP-inhibited cAMP PDE (cGI-PDE) family, such as PDE3A, are characterized by high affinity for cAMP and cGMP and competitive inhibition of their cAMP hydrolytic activity by cGMP and certain positive inotropic agents (Meacci et al., 1992 [PubMed 1315035]).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: cAMP phosphodiesterase, myocardial cGMP-inhibited; CGI-PDE A; cGMP-inhibited 3',5'-cyclic phosphodiesterase A; Cyclic GMP-inhibited phosphodiesterase A; phosphodiesterase 3A, cGMP-inhibited

Gene Aliases: CGI-PDE; CGI-PDE A; CGI-PDE-A; HTNB; PDE3A

UniProt ID: (Human) Q14432

Entrez Gene ID: (Human) 5139

Molecular Function: phosphodiesterase