Proteintech

PDE6A Polyclonal Antibody

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PDE6A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 21200-1-AP (PDE6A Antibody) at dilution of 1:50 (under 40x lens).

PDE6A Antibody in Immunohistochemistry (IHC) — IF stain of PDE6a antibody with retinal tissue by a customer. (PDE6a is in red and nuclei are blue).

PDE6A Antibody in Western Blot (WB) — WB results of PDE6A antibody (21200-1-AP) with WT mouse Eye and Prph2 (Rds) mutant mouse Eye (Negative control). Courtesy of Seongjin Seo, PhD, University of Iowa College of Medicine.

Product Details

21200-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:200	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PDE6A Fusion Protein Ag15562 (1-90 aa encoded by BC035909)
Conjugate	Unconjugated
Form	Liquid
Concentration	0.19 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MGEVTAEEV EKFLDSNIGF AKQYYNLHYR AKLISDLLGA KEAAVDFSNY HSPSSMEESE IIFDLLRDFQ ENLQTEKCIF NVMKKLCFLL Q (1-90 aa encoded by BC035909)

Target Information

This gene encodes the cyclic-GMP (cGMP)-specific phosphodiesterase 6A alpha subunit, expressed in cells of the retinal rod outer segment. The phosphodiesterase 6 holoenzyme is a heterotrimer composed of an alpha, beta, and two gamma subunits. cGMP is an important regulator of rod cell membrane current, and its dynamic concentration is established by phosphodiesterase 6A cGMP hydrolysis and guanylate cyclase cGMP synthesis. The protein is a subunit of a key phototransduction enzyme and participates in processes of transmission and amplification of the visual signal. Mutations in this gene have been identified as one cause of autosomal recessive retinitis pigmentosa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Luteinizing hormone signaling phosphorylates and activates the cyclic GMP phosphodiesterase PDE5 in mouse ovarian follicles, contributing an additional component to the hormonally induced decrease in cyclic GMP that reinitiates meiosis.

Developmental biology

Egbert JR,Yee SP,Jaffe LA

Thu Mar 01 00:00:00 EST 2018

Mouse models of human ocular disease for translational research.

PloS one

Krebs MP,Collin GB,Hicks WL,Yu M,Charette JR,Shi LY,Wang J,Naggert JK,Peachey NS,Nishina PM

Fri Oct 13 00:00:00 EDT 2017

Ocular toxicity of AUY922 in pigmented and albino rats.

Toxicology and applied pharmacology

Roman D,VerHoeve J,Schadt H,Vicart A,Walker UJ,Turner O,Richardson TA,Wolford ST,Miller PE,Zhou W,Lu H,Akimov M,Kluwe W

Sat Oct 15 00:00:00 EDT 2016

Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome.

Proceedings of the National Academy of Sciences of the United States of America

Datta P,Allamargot C,Hudson JS,Andersen EK,Bhattarai S,Drack AV,Sheffield VC,Seo S

Tue Aug 11 00:00:00 EDT 2015

Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome.

Proceedings of the National Academy of Sciences of the United States of America

Datta P,Allamargot C,Hudson JS,Andersen EK,Bhattarai S,Drack AV,Sheffield VC,Seo S

Tue Aug 11 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: cGMP phosphodiesterase alpha; cGMP phosphodiesterase alpha subunit; GMP-PDE alpha; PDE V-B1; phosphodiesterase 6A, cGMP-specific, rod, alpha; Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha; rod photoreceptor cGMP phosphodiesterase alpha subunit

Gene Aliases: CGPR-A; nmf282; PDE6A; PDEA; RP43

UniProt ID: (Human) P16499

Entrez Gene ID: (Human) 5145, (Mouse) 225600, (Rat) 307401

Molecular Function: phosphodiesterase

Function(s)

Wnt signaling pathway, calcium modulating pathway visual perception rhodopsin mediated signaling pathway regulation of rhodopsin mediated signaling pathway GMP metabolic process regulation of cytosolic calcium ion concentration signal transduction photoreceptor cell maintenance response to stimulus retina development in camera-type eye biological_process

Process(es)

metal ion binding 3',5'-cyclic-GMP phosphodiesterase activity 3',5'-cyclic-nucleotide phosphodiesterase activity phosphoric diester hydrolase activity hydrolase activity molecular_function

PDE6A Polyclonal Antibody

Product Details

21200-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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