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Invitrogen
PDE6A Polyclonal Antibody
Product Details
PA1-720
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Sheep
Published species
Bovine,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: M(1) G E V T A E E V E K F L D S N(16) C
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2268019
Product Specific Information
PA1-720 detects phosphodiesterase 6 alpha (PDE6 alpha) from sheep retinal extract and bovine rod outer segment membranes as well as purified bovine PDE6.
PA1-720 has been successfully used in Western blot and immunofluoresence procedures. By Western blot, this antibody detects an ~90 kDa protein representing PDE6 alpha from sheep retinal extract. A higher molecular weight band is sometimes observed using sheep retinal extracts.
The PA1-720 immunizing peptide corresponds to amino acid residues 1-16 from human PDE6 alpha. This sequence is completely conserved between human, mouse and bovine and 94% conserved in canine. PA1-720 immunizing peptide (Cat. # PEP-148) is available for use in neutralization and control experiments.
Target Information
This gene encodes the cyclic-GMP (cGMP)-specific phosphodiesterase 6A alpha subunit, expressed in cells of the retinal rod outer segment. The phosphodiesterase 6 holoenzyme is a heterotrimer composed of an alpha, beta, and two gamma subunits. cGMP is an important regulator of rod cell membrane current, and its dynamic concentration is established by phosphodiesterase 6A cGMP hydrolysis and guanylate cyclase cGMP synthesis. The protein is a subunit of a key phototransduction enzyme and participates in processes of transmission and amplification of the visual signal. Mutations in this gene have been identified as one cause of autosomal recessive retinitis pigmentosa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cGMP phosphodiesterase alpha subunit; cGMP phosphodiesterase alpha subunit (EC 3.1.4.35); cyclic GMP phosphodiesterase alpha subunit; GMP phosphodiesterase alpha subunit; GMP-PDE alpha; PDE V-B1; phosphodiesterase 6A, cGMP-specific, rod, alpha; phosphodiesterase, cyclic GMP (rod receptor), alpha polypeptide; Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha; rod photoreceptor cGMP phosphodiesterase alpha subunit; unnamed protein product
Gene Aliases: CGPR-A; PDE6A; PDEA; RP43
UniProt ID: (Bovine) P11541, (Human) P16499
Entrez Gene ID: (Sheep) 101122024, (Bovine) 281973, (Human) 5145
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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