Invitrogen

PDE6A Polyclonal Antibody

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PDE6A Antibody in Western Blot (WB) — Western blot of PDE6 alpha on sheep retinal extracts using Product # PA1-720.

PDE6A Antibody in Immunohistochemistry (IHC) — Published figure using PDE6A polyclonal antibody (Product # PA1-720) in Immunohistochemistry

Product Details

PA1-720

Applications	Tested Dilution	Publications
Western Blot (WB)	1-2 µg/mL	View 13 publications 13 publications
Immunocytochemistry (ICC/IF)	Assay-dependent	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Bovine, Human, Sheep
Published species	Bovine, Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic Peptide: M(1) G E V T A E E V E K F L D S N(16) C View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 1mg/mL BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2268019

Product Specific Information

PA1-720 detects phosphodiesterase 6 alpha (PDE6 alpha) from sheep retinal extract and bovine rod outer segment membranes as well as purified bovine PDE6.

PA1-720 has been successfully used in Western blot and immunofluoresence procedures. By Western blot, this antibody detects an ~90 kDa protein representing PDE6 alpha from sheep retinal extract. A higher molecular weight band is sometimes observed using sheep retinal extracts.

The PA1-720 immunizing peptide corresponds to amino acid residues 1-16 from human PDE6 alpha. This sequence is completely conserved between human, mouse and bovine and 94% conserved in canine. PA1-720 immunizing peptide (Cat. # PEP-148) is available for use in neutralization and control experiments.

Target Information

This gene encodes the cyclic-GMP (cGMP)-specific phosphodiesterase 6A alpha subunit, expressed in cells of the retinal rod outer segment. The phosphodiesterase 6 holoenzyme is a heterotrimer composed of an alpha, beta, and two gamma subunits. cGMP is an important regulator of rod cell membrane current, and its dynamic concentration is established by phosphodiesterase 6A cGMP hydrolysis and guanylate cyclase cGMP synthesis. The protein is a subunit of a key phototransduction enzyme and participates in processes of transmission and amplification of the visual signal. Mutations in this gene have been identified as one cause of autosomal recessive retinitis pigmentosa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Regulation of rod photoreceptor function by farnesylated G-protein γ-subunits.

PloS one

Kolesnikov AV,Lobysheva E,Gnana-Prakasam JP,Kefalov VJ,Kisselev OG

PA1-720 was used in Western Blotting to conclude that, while farnesylated Gγ subunits are functionally active and largely interchangeable in supporting rod phototransduction, replacement of retina-specific Gγ isoforms by the ubiquitous Gγ11 affects the ability of rods to adapt to background light.

Wed Aug 10 00:00:00 EDT 2022

Essential role of the chaperonin CCT in rod outer segment biogenesis.

Investigative ophthalmology & visual science

Sinha S,Belcastro M,Datta P,Seo S,Sokolov M

PA1-720 was used in immunocytochemistry to study the biogenesis of rod outer segments and the indespensible role of chaperonin containing t-complex protein-1

Thu May 22 00:00:00 EDT 2014

Essential role of the chaperonin CCT in rod outer segment biogenesis.

Investigative ophthalmology & visual science

Sinha S,Belcastro M,Datta P,Seo S,Sokolov M

PA1-720 was used in immunocytochemistry to study the biogenesis of rod outer segments and the indespensible role of chaperonin containing t-complex protein-1

Thu May 22 00:00:00 EDT 2014

Leukemia inhibitory factor coordinates the down-regulation of the visual cycle in the retina and retinal-pigmented epithelium.

The Journal of biological chemistry

Chucair-Elliott AJ,Elliott MH,Wang J,Moiseyev GP,Ma JX,Politi LE,Rotstein NP,Akira S,Uematsu S,Ash JD

PA1-720 was used in western blot to study the role of leukemia inhibitory factor on the visual cycle in the retina and retinal-pigmented epithelium

Fri Jul 13 00:00:00 EDT 2012

Effect of the ILE86TER mutation in the γ subunit of cGMP phosphodiesterase (PDE6) on rod photoreceptor signaling.

Cellular signalling

Tsang SH,Woodruff ML,Lin CS,Jacobson BD,Naumann MC,Hsu CW,Davis RJ,Cilluffo MC,Chen J,Fain GL

Published figure using PDE6A polyclonal antibody (Product # PA1-720) in Western Blot

Sun Jan 01 00:00:00 EST 2012

Function of the asparagine 74 residue of the inhibitory γ-subunit of retinal rod cGMP-phophodiesterase (PDE) in vivo.

Cellular signalling

Tsang SH,Woodruff ML,Hsu CW,Naumann MC,Cilluffo M,Tosi J,Lin CS

Published figure using PDE6A polyclonal antibody (Product # PA1-720) in Western Blot

Sat Oct 01 00:00:00 EDT 2011

In vitro interaction of tubulin with the photoreceptor cGMP phosphodiesterase gamma-subunit.

Neuroscience letters

Chu UB,Song J,Mavlyutov TA,Guo LW

PA1-720 was used in immunohistochemistry to study the interaction of the photoreceptor cGMP phosphodiesterase with tubulin.

Mon Oct 04 00:00:00 EDT 2010

Replacing the rod with the cone transducin subunit decreases sensitivity and accelerates response decay.

The Journal of physiology

Chen CK,Woodruff ML,Chen FS,Shim H,Cilluffo MC,Fain GL

PA1-720 was used in western blot to investigate the mechanism why cone vision is less sensitive than rod vision

Wed Sep 01 00:00:00 EDT 2010

High levels of retinal membrane docosahexaenoic acid increase susceptibility to stress-induced degeneration.

Journal of lipid research

Tanito M,Brush RS,Elliott MH,Wicker LD,Henry KR,Anderson RE

PA1-720 was used in western blot to study the effect of retinal membrane docosahexaenoic acid on stress-induced retinal degeneration in mice.

Fri May 01 00:00:00 EDT 2009

Phototransduction in a transgenic mouse model of Nougaret night blindness.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Moussaif M,Rubin WW,Kerov V,Reh R,Chen D,Lem J,Chen CK,Hurley JB,Burns ME,Artemyev NO

Published figure using PDE6A polyclonal antibody (Product # PA1-720) in Western Blot

Wed Jun 21 00:00:00 EDT 2006

The inhibitory gamma subunit of the rod cGMP phosphodiesterase binds the catalytic subunits in an extended linear structure.

The Journal of biological chemistry

Guo LW,Muradov H,Hajipour AR,Sievert MK,Artemyev NO,Ruoho AE

PA1-720 was used in western blot to study the interaction between the inhibitory gamma subunit and the catalytic subunit of the rod cGMP phosphodiesterase.

Fri Jun 02 00:00:00 EDT 2006

GAP-independent termination of photoreceptor light response by excess gamma subunit of the cGMP-phosphodiesterase.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Tsang SH,Woodruff ML,Chen CK,Yamashita CY,Cilluffo MC,Rao AL,Farber DB,Fain GL

PA1-720 was used in western blot to study the effect of the cGMP-phosphodiesterase gamma subunit on photoreceptor light response termination.

Wed Apr 26 00:00:00 EDT 2006

Transducin activation state controls its light-dependent translocation in rod photoreceptors.

The Journal of biological chemistry

Kerov V,Chen D,Moussaif M,Chen YJ,Chen CK,Artemyev NO

PA1-720 was used in western blot to investigate the role of the GTP-hydrolysis on Gtalpha for light-dependent translocation in rod photoreceptors

Fri Dec 09 00:00:00 EST 2005

Asymmetric interaction between rod cyclic GMP phosphodiesterase gamma subunits and alphabeta subunits.

The Journal of biological chemistry

Guo LW,Grant JE,Hajipour AR,Muradov H,Arbabian M,Artemyev NO,Ruoho AE

PA1-720 was used in western blot to demonstrate a new model of interaction between the subunits of the rod cyclic GMP phosphodiesterase holoenzyme.

Fri Apr 01 00:00:00 EST 2005

Enhancement of phototransduction g protein-effector interactions by phosphoinositides.

The Journal of biological chemistry

He F,Mao M,Wensel TG

PA1-720 was used in western blot to study the modulating mechanism for phototransduction efficiency.

Fri Mar 05 00:00:00 EST 2004

Identification of the gamma subunit-interacting residues on photoreceptor cGMP phosphodiesterase, PDE6alpha '.

The Journal of biological chemistry

Granovsky AE,Artemyev NO

PA1-720 was used in western blot to investigate the regulation of photoreceptor cGMP phosphodiesteras 6 activity

Fri Dec 29 00:00:00 EST 2000

Bioinformatics

Protein Aliases: cGMP phosphodiesterase alpha subunit; cGMP phosphodiesterase alpha subunit (EC 3.1.4.35); cyclic GMP phosphodiesterase alpha subunit; GMP phosphodiesterase alpha subunit; GMP-PDE alpha; PDE V-B1; phosphodiesterase 6A, cGMP-specific, rod, alpha; phosphodiesterase, cyclic GMP (rod receptor), alpha polypeptide; Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha; rod photoreceptor cGMP phosphodiesterase alpha subunit; unnamed protein product

Gene Aliases: CGPR-A; PDE6A; PDEA; RP43

UniProt ID: (Bovine) P11541, (Human) P16499

Entrez Gene ID: (Sheep) 101122024, (Bovine) 281973, (Human) 5145

Molecular Function: phosphodiesterase