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Invitrogen
PDE6B Polyclonal Antibody
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FIGURE: 1 / 21
PDE6B Antibody (PA1-722) in ICC/IF
Immunofluorescence analysis of PDE6 beta was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PDE6B Rabbit Polyclonal Antibody (Product # PA1-722) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (... View More
Product Details
PA1-722
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Bovine,
C. elegans,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptides: H(20) Q Y F G (K/R) K L S P E N V A G A C(36)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-722 detects phosphodiesterase 6 beta (PDE6 beta) from mouse retinal extracts.
PA1-722 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~90 kDa protein representing PDE6 beta from mouse retinal extracts.
The PA1-722 immunizing peptides correspond to amino acid residues 20-36 from mouse and bovine PDE6 beta protein. These peptides (Cat. # PEP-183) are available for use in neutralization and control experiments.
Target Information
The second messengers cAMP and cGMP are key regulatory molecules that are involved in a wide variety of signal transduction pathways. Levels of cAMP and cGMP are regulated by their rate of synthesis by nucleotide cyclases and by their rate of hydrolysis by cyclic nucleotide phosphodiesterases (PDEs). PDEs form a superfamily of enzymes that catalyze the conversion of 3-prime, 5-prime-cyclic nucleotides to the corresponding nucleoside 5-prime-monophosphates. PDE6 is the effector enzyme in the G protein-mediated signal transduction cascade in the visual system. There are five different subunits consisting of rod and cone specific catalytic subunits: alpha' (Cone), alpha (Rod), and beta (Rod), the inhibitory subunit gamma, and subunit delta of unknown function (which likely interacts with many other proteins besides the PDE6 family). The catalytic core of the PDE6 system is comprised of alpha'/alpha' homodimers in the cone and alpha/beta heterodimers in the rod. The C-terminus of both the catalytic and inhibitory subunits is modified by methylation, myristyolation and prenylation which have been shown to be critical for proper complex assembly and membrane association.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cGMP-phosphodiesterase beta-subunit; GMP-PDE beta; phosphodiesterase 6B, cGMP-specific, rod, beta; phosphodiesterase, cGMP, rod receptor, beta polypeptide; retinal degeneration 1; rod cGMP-phosphodiesterase beta-subunit; Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta
Gene Aliases: CSNB3; CSNBAD2; Mpb; PDE6B; PDEB; r; rd; rd-1; rd1; rd10; RP40
UniProt ID: (Human) P35913, (Mouse) P23440
Entrez Gene ID: (Human) 5158, (Mouse) 18587
Wnt signaling pathway, calcium modulating pathway
visual perception
phototransduction, visible light
rhodopsin mediated signaling pathway
regulation of rhodopsin mediated signaling pathway
GMP metabolic process
regulation of cytosolic calcium ion concentration
retina development in camera-type eye
signal transduction
detection of light stimulus
response to stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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