Invitrogen

PDGF-A Polyclonal Antibody

View all (24) PDGF-A antibodies

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Questions & Answers

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PDGF-A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded PDGF-A in human colon tissues sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with PDGF-A polyclonal antibody (Product # PA5-99405) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

PDGF-A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded PDGF-A in human uterus tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with PDGF-A polyclonal antibody (Product # PA5-99405) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

PDGF-A Antibody in Western Blot (WB) — Western blot analysis of PDGF-A in various samples (Lane 1: mouse brain lysate, Lane 2: mouse muscle lysate, Lane 3: rat spleen lysate, Lane 4: rat liver lysate, Lane 5: rat liver lysates treated with blocking peptide). Samples were incubated with PDGF-A polyclonal antibody (Product # PA5-99405).

PDGF-A Antibody in Western Blot (WB) — Western blot analysis of PDGF-A in various samples (Lane 1: PC12, treated with blocking peptide
Lane 2: PC12
Lane 3: 3T3). Samples were incubated with PDGF-A polyclonal antibody (Product # PA5-99405).

Product Details

PA5-99405

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human PDGFA(Accession P04085), corresponding to amino acid residues R144-L171. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2818338

Target Information

This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: PDGF A-chain; PDGF subunit A; PDGF-1; platelet derived growth factor, alpha; Platelet-derived growth factor A chain; platelet-derived growth factor A-chain; platelet-derived growth factor alpha chain; Platelet-derived growth factor alpha polypeptide; Platelet-derived growth factor subunit A

Gene Aliases: PDGF-A; PDGF1; PDGFA; PDGFACP; Rpa1

UniProt ID: (Human) P04085, (Rat) P28576, (Mouse) P20033

Entrez Gene ID: (Human) 5154, (Rat) 25266, (Mouse) 18590

Molecular Function: growth factor

MAPK cascade angiogenesis response to hypoxia cell activation hair follicle development positive regulation of mesenchymal cell proliferation platelet degranulation transforming growth factor beta receptor signaling pathway cell-cell signaling positive regulation of cell proliferation response to wounding organ morphogenesis response to inorganic substance negative regulation of phosphatidylinositol biosynthetic process negative regulation of platelet activation regulation of phosphatidylinositol 3-kinase signaling positive regulation of phosphatidylinositol 3-kinase signaling regulation of smooth muscle cell migration cell projection assembly actin cytoskeleton organization extracellular matrix organization positive regulation of cell migration positive regulation of protein autophosphorylation response to estradiol response to retinoic acid regulation of actin cytoskeleton organization positive regulation of metanephric mesenchymal cell migration by platelet-derived growth factor receptor-beta signaling pathway wound healing response to drug positive regulation of MAP kinase activity positive regulation of MAPK cascade positive regulation of GTPase activity skin development positive regulation of DNA replication phosphatidylinositol phosphorylation platelet-derived growth factor receptor signaling pathway phosphatidylinositol-mediated signaling positive regulation of fibroblast proliferation lung alveolus development inner ear development regulation of peptidyl-tyrosine phosphorylation negative chemotaxis positive regulation of cell division positive regulation of protein kinase B signaling regulation of branching involved in salivary gland morphogenesis by epithelial-mesenchymal signaling positive regulation of ERK1 and ERK2 cascade regulation of glomerular mesangial cell proliferation embryonic lung development regulation of DNA biosynthetic process blood coagulation response to organic substance digestive tract development multicellular organism development bone development

Process(es)

Ras guanyl-nucleotide exchange factor activity platelet-derived growth factor receptor binding protein binding collagen binding growth factor activity protein homodimerization activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein heterodimerization activity platelet-derived growth factor binding