Invitrogen

PDGF-D Polyclonal Antibody

3 Published Figures

6 References

View all (7) PDGF-D antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

PDGF-D Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PDGF-D showing staining in the cytoplasm of paraffin-embedded human heart tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- PDGF-D Polyclonal Antibody (Product # 40-2100) diluted in 3% BSA-PBS at a dilution of 1:50 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

PDGF-D Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of Mouse Brain (Lane 1), A549 (Lane 2), SH-SY5Y (Lane 3) and THP-1 (Lane 4). The blots were probed with Anti-PDGFD Rabbit Polyclonal Antibody (Product # 40-2100, 1-3 µg/mL) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). A 42 kDa band corresponding to PDGFD was observed across cell lines and tissues tested. An extra band at ~ 35 kDa was observed in cell lines alone.Known quantity of protein samples were electrophoresed using Novex® NuPAGE®12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane by iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

PDGF-D Antibody — Published figure using PDGF-D polyclonal antibody (Product # 40-2100)

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50

View 1 publication 1 publication

Western Blot (WB)

Assay Dependent

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Product Specifications

Species

Human, Mouse, Rat

Published species

Artificial Control, Human, Rat

Expression System

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the C-terminal region of the human PDGF-D

Conjugate

Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

RRID

AB_2533455

Target Information

The platelet-derived growth factor (PDGF) family consists of four members; PDGF-A, PDGF-B, PDGF-C and PDGF-D (spinal cord-derived growth factor-B or iris-expressed growth factor). PDGF members form disulphide-bonded dimeric isoforms, which are important for growth, survival and function in several types of connective tissue cells. Their biological effects are mediated via two tyrosine kinase receptors, PDGFR-a and PDGFR-b, and PDGF-mediated signaling is critical for development of many organ systems. PDGF-D has a two-domain structure similar to PDGF-C and is secreted as a disulphide-linked homodimer, PDGF-DD. PDGF-D induces cellular transformation and promotes tumor growth by accelerating the proliferation rate of tumor cells and by stimulation of tumor neovascularization. PDGF-D may play a role in the development of brain tumors. The potential oncogenic activity of PDGF-DD may be important for the development and/or progression of prostate cancer. PDGF-D is expressed in fibroblastic adventitial cells, cultured endothelial cells and in a variety of tumor cell lines including those derived from ovarian, renal and lung cancers, as well as from astrocytomas and medulloblastomas. PDGF-D is expressed in the human kidney.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Iris-expressed growth factor; PDGF-D; PDGFD latent form; PDGFD receptor-binding form; Platelet-derived growth factor D; Platelet-derived growth factor D, latent form; Platelet-derived growth factor D, receptor-binding form; platelet-derived growth factor, D polypeptide; SCDGF-B; spinal cord derived growth factor B; Spinal cord-derived growth factor B; spinal cord-derived growth factor-B; spinal-cord derived growth factor-B protein

Gene Aliases: 1110003I09Rik; IEGF; MSTP036; PDGFD; rSCDGF-B; SCDGF-B; SCDGFB; UNQ1899/PRO4345

UniProt ID: (Human) Q9GZP0, (Rat) Q9EQT1, (Mouse) Q925I7

Entrez Gene ID: (Human) 80310, (Rat) 66018, (Mouse) 71785

Molecular Function: growth factor signaling molecule