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PDGFRA Antibody (710169) in ICC/IF
Immunofluorescent analysis of PDGFR alpha in serum-starved HeLa cells treated with insulin (100 ng/mL, 15 min) using a PDGFR alpha Recombinant Rabbit Superclonal™ Antibody (Product # 710169) followed by detection using an Alexa Fluor 488-conjugated Goat anti-Rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing cytoplasmic and nuclear membrane localization of PDGFR-alpha.
Product Details
710169
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
C. elegans,
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to amino acids 259-436 of human platelet-derived growth factor receptor - alpha chain
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
PDGFRA is a cell surface receptor tyrosine kinase which binds members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Alpha platelet-derived growth factor receptor; Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a; CD140a antigen; MGC74795; OTTHUMP00000218656; PDGF A-chain; PDGF Receptor alpha; PDGF subunit A; PDGF-1; PDGF-R-alpha; PDGFR-2; pdgfr-a; PDGFRA/BCR fusion; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor 2; Platelet-derived growth factor receptor alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alpha fusion protein
Gene Aliases: CD140A; GAS9; PDGFR-2; PDGFR2; PDGFRA; RHEPDGFRA
UniProt ID: (Human) P16234
Entrez Gene ID: (Human) 5156
protein kinase activity
transmembrane receptor protein tyrosine kinase activity
platelet-derived growth factor alpha-receptor activity
vascular endothelial growth factor-activated receptor activity
Ras guanyl-nucleotide exchange factor activity
platelet-derived growth factor receptor binding
protein binding
ATP binding
vascular endothelial growth factor binding
protein homodimerization activity
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
platelet-derived growth factor binding
transmembrane signal receptor
MAPK cascade
luteinization
cell activation
positive regulation of cytosolic calcium ion concentration
positive regulation of cell proliferation
negative regulation of platelet activation
positive regulation of phospholipase C activity
regulation of phosphatidylinositol 3-kinase signaling
positive regulation of phosphatidylinositol 3-kinase signaling
viral process
peptidyl-tyrosine phosphorylation
positive regulation of cell migration
cellular response to reactive oxygen species
platelet-derived growth factor receptor-alpha signaling pathway
positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway
wound healing
positive regulation of GTPase activity
positive regulation of phosphatidylinositol 3-kinase activity
positive regulation of DNA replication
protein autophosphorylation
phosphatidylinositol phosphorylation
platelet-derived growth factor receptor signaling pathway
phosphatidylinositol-mediated signaling
positive regulation of fibroblast proliferation
embryonic digestive tract morphogenesis
embryonic cranial skeleton morphogenesis
embryonic skeletal system morphogenesis
regulation of chemotaxis
cardiac myofibril assembly
cell chemotaxis
retina vasculature development in camera-type eye
positive regulation of ERK1 and ERK2 cascade
platelet aggregation
metanephric glomerular capillary formation
regulation of actin cytoskeleton reorganization
regulation of mesenchymal stem cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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