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Invitrogen
PDHA1A Polyclonal Antibody
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FIGURE: 1 / 1
PDHA1A Antibody (PA5-72684) in WB
Western blot analysis of PDHA1A in various lysates. Samples were incubated with PDHA1A polyclonal antibody (Product # PA5-72684) using a dilution of 1:1,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: Zebrafish lysate; Lane 2: ZF4 whole cell lysate. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Product Details
PA5-72684
Product Specifications
Species Reactivity
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 233~263 amino acids from the Central region of Zebrafish PDHA1
Conjugate
Form
Liquid
Concentration
0.97 mg/mL
Amount
388 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: pdha1b; PDHE1-A type I; PHE1A; pyruvate dehydrogenase (lipoamide) alpha 1; pyruvate dehydrogenase (lipoamide) alpha 1a; pyruvate dehydrogenase E1 alpha 1 subunit a
Gene Aliases: im:6895726; pdha1; wu:fd18b01; wu:fo96f03; zgc:73271; zgc:86692
Entrez Gene ID: (Zebrafish) 406702
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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