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FIGURE: 1 / 2
PDHX Antibody (PA5-110091) in WB
Western blot analysis of PDHX in extracts of various cell lines. Samples were incubated with PDHX Polyclonal antibody (Product # PA5-110091) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
PA5-110091
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PDHX (NP_003468.2).
Conjugate
Form
Liquid
Concentration
2.8 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2855502
Product Specific Information
Immunogen sequence: MAASWRLGCD PRLLRYLVGF PGRRSVGLVK GALGWSVSRG ANWRWFHSTQ WLRGDPIKIL MPSLSPTMEE GNIVKWLKKE GEAVSAGDAL CEIETDKAVV TLDASDDGIL AKIVVEEGSK NIRLGSLIGL IVEEGEDWKH VEIPKDVGPP PPVSKPSEPR PSPEPQISIP VKKEHIPGTL RFRLSPAARN ILEKHSLDAS QGTATGPRGI FTKEDALKLV QLKQTGKITE SRPTPAPTAT PTAPSPLQAT AGPSYPRPVI PPVSTPGQPN AVGTFTEIPA SNIRRVIAKR LTESKSTVPH
Target Information
The PDHX gene encodes component X of the pyruvate dehydrogenase (PDH) complex. For a detailed description of the pyruvate dehydrogenase complex. The mammalian PDH complex differs from that in E. coli and from the other mammalian alpha-keto acid dehydrogenases by the presence of a 53-kDa protein called protein X. Component X binds to the E3 component of the PDH complex (Robinson et al., 1990 ; Aral et al., 1997 ).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: component X; Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex; dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex (E3-binding protein) (proX); E3-binding protein; E3BP; IPF1; lipoyl-containin; Lipoyl-containing pyruvate dehydrogenase complex component X; proX; pyruvate dehydrogenase; pyruvate dehydrogenase complex, component X; pyruvate dehydrogenase complex, E3-binding protein subunit; pyruvate dehydrogenase complex, lipoyl-containing component X; Pyruvate dehydrogenase protein X component, mitochondrial
Gene Aliases: Ac1164; AI481367; DLDBP; E3BP; OPDX; PDHX; PDX1; proX
UniProt ID: (Human) O00330, (Mouse) Q8BKZ9
Entrez Gene ID: (Human) 8050, (Rat) 311254, (Mouse) 27402
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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