Invitrogen

PDIA6 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

3 Published Figures

4 References

View all (15) PDIA6 antibodies

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Datasheet

Protocols

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PDIA6 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of PDIA6 (P5) was performed using 70% confluent log phase A-549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PDIA6 Rabbit Polyclonal Antibody (Product # PA3-008) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

PDIA6 Antibody in Western Blot (WB) — Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of A549 (Lane 1), A-431 (Lane 2), HeLa (Lane 3), MCF7 (Lane 4), K-562 (Lane 5), Caco-2 (Lane 6), Hep G2 (Lane 7) and COLO 205 (Lane 8). The blot was probed with Anti-P5 Rabbit Polyclonal Antibody (Product # PA3-008, 1:250 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 48 kDa band corresponding to P5 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

PDIA6 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with PDIA6 siRNA and decrease in signal was observed in Western Blot using Anti- PDIA6 polyclonal Antibody (Product # PA3-008). {KD}

Product Details

PA3-008

Applications	Tested Dilution	Publications
Western Blot (WB)	1:250	View 4 publications 4 publications
Immunocytochemistry (ICC/IF)	1:250	-

Product Specifications
Species Reactivity	Human
Published species	Human, Pig
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to residues D(476) I D L S D V E L D D L G K D E L(492) of human P5.
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	whole serum
Contains	0.01% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2161009

Product Specific Information

PA3-008 detects P5 protein in human samples.

PA3-008 has successfully been used in Western blot procedures. By Western blot, this antibody detects an ~50 kDa protein representing P5 in human fibrosarcoma HT 1080 cell lysate.

The PA3-008 immunogen is a synthetic peptide corresponding to residues D(476) I D L S D V E L D D L G K D E L(492) of human P5. This peptide (Cat. # PEP-224) is available for use in neutralization and control experiments.

Target Information

P5 is a novel protein that was originally identified in humans. Functionally, P5 has been shown to have peptide binding ability and chaperone activity specific to certain proteins. P5 shares several structural similarities to PDI, an important protein of the ER. The deduced amino acid sequence of P5 shows that it contains the ER retention signal KDEL. P5 also contains two CXXC-like motifs, a motif responsible for oxidoreductase activity. These two CXXC motifs are found on the N- and C-terminus of the protein, one at each end. Studies suggest that the N-terminal CXXC motif is more important than the second for isomerase activity. Contrary to most of the PDI-like proteins, P5 is not stress-inducible.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ERp18 regulates activation of ATF6α during unfolded protein response.

The EMBO journal

Oka OB,van Lith M,Rudolf J,Tungkum W,Pringle MA,Bulleid NJ

PA3-008 was used in Western Blotting to demonstrate that ERp18 monitors ATF6α ER quality control to ensure optimal processing following trafficking to the Golgi.

Thu Aug 01 00:00:00 EDT 2019

Transmembrane thioredoxin-related protein TMX1 is reversibly oxidized in response to protein accumulation in the endoplasmic reticulum.

FEBS open bio

Matsuo Y,Hirota K

Published figure using PDIA6 polyclonal antibody (Product # PA3-008) in Western Blot

Wed Nov 01 00:00:00 EDT 2017

Cyclophilin C Participates in the US2-Mediated Degradation of Major Histocompatibility Complex Class I Molecules.

PloS one

Chapman DC,Stocki P,Williams DB

PA3-008 was used in western blot to study how cyclophorin c interacts with US2-mediated degradation of MHC class 1 molecules

Mon Jul 25 00:00:00 EDT 2016

Protein disulfide isomerase-P5, down-regulated in the final stage of boar epididymal sperm maturation, catalyzes disulfide formation to inhibit protein function in oxidative refolding of reduced denatured lysozyme.

Biochimica et biophysica acta

Akama K,Horikoshi T,Sugiyama A,Nakahata S,Akitsu A,Niwa N,Intoh A,Kakui Y,Sugaya M,Takei K,Imaizumi N,Sato T,Matsumoto R,Iwahashi H,Kashiwabara S,Baba T,Nakamura M,Toda T

PA3-008 was used in western blot to investigate the physiological roles of PDIA3 and PDI-P5 in mammalian spermiogenesis

Tue Jun 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: Endoplasmic reticulum protein 5; ER protein 5; protein disulfide isomerase family A, member 6; Protein disulfide isomerase P5; protein disulfide isomerase-associated 6; protein disulfide isomerase-related protein; Protein disulfide-isomerase A6; thioredoxin domain containing 7 (protein disulfide isomerase); Thioredoxin domain-containing protein 7

Gene Aliases: ERP5; P5; PDIA6; TXNDC7

UniProt ID: (Human) Q15084

Entrez Gene ID: (Human) 10130

Molecular Function: chaperone

Function(s)

protein folding response to endoplasmic reticulum stress IRE1-mediated unfolded protein response apoptotic cell clearance cell redox homeostasis

Process(es)

protein disulfide isomerase activity protein binding

PDIA6 Polyclonal Antibody

Product Details

PA3-008

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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