Invitrogen

PDK1 Polyclonal Antibody

View all (18) PDK1 antibodies

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Product Details

PA5-106712

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human PDK1(Accession Q15118), corresponding to amino acid residues L102-V152.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2854380

Product Specific Information

Antibody detects endogenous levels of total PDHK1.

Target Information

PDK1 (pyruvate dehydrogenase kinase, isozyme 1) gene, located on chromosome 2q31.1, encodes a member of the pyruvate dehydrogenase kinase family. This kinase plays a crucial role in the regulation of glucose metabolism by phosphorylating and inactivating the pyruvate dehydrogenase complex (PDC), which converts pyruvate to acetyl-CoA, linking glycolysis to the Krebs cycle. Structurally, the PDK1 protein contains a kinase domain that is essential for its enzymatic activity. PDK1 acts as a gatekeeper of glucose oxidation and is tightly regulated by various metabolic signals, including insulin and hypoxia. Dysregulation of PDK1 activity has been implicated in metabolic disorders such as diabetes and cancer, where it contributes to altered cellular energy homeostasis and metabolic reprogramming.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 3-phosphoinositide dependent protein kinase-1; 3-phosphoinositide-dependent protein kinase 1; 3-phosphoinositide-dependent protein kinase 2 pseudogene; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial; hPDK1; isoenzyme 1; kinase isoenzyme 1; lipoamide; PDH kinase 1; PDK p48; PkB kinase like gene 1; pyruvate dehydrogenase; pyruvate dehydrogenase kinase 1; Pyruvate dehydrogenase kinase isoform 1; pyruvate dehydrogenase kinase, isoenzyme 1; pyruvate dehydrogenase kinase, isozyme 1

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Gene Aliases: B830012B01; D530020C15Rik; PDK1; PDPK1; PDPK2; PDPK2P; PRO0461

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UniProt ID: (Human) O15530, (Mouse) Q8BFP9

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Entrez Gene ID: (Human) 5170, (Rat) 116551, (Mouse) 228026

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Function(s)
protein serine/threonine kinase activity 3-phosphoinositide-dependent protein kinase activity insulin receptor binding protein binding ATP binding phospholipase activator activity kinase activity protein kinase binding phospholipase binding protein kinase activity pyruvate dehydrogenase (acetyl-transferring) kinase activity protein complex binding protein homodimerization activity protein heterodimerization activity nucleotide binding transferase activity non-receptor serine/threonine protein kinase
Process(es)
stimulatory C-type lectin receptor signaling pathway type B pancreatic cell development transcription, DNA-templated regulation of transcription, DNA-templated protein phosphorylation negative regulation of protein kinase activity hyperosmotic response epidermal growth factor receptor signaling pathway positive regulation of phospholipase activity regulation of endothelial cell migration negative regulation of cardiac muscle cell apoptotic process cell migration peptidyl-serine phosphorylation peptidyl-threonine phosphorylation calcium-mediated signaling actin cytoskeleton organization platelet activation negative regulation of transforming growth factor beta receptor signaling pathway T cell costimulation activation of protein kinase B activity cellular response to insulin stimulus negative regulation of toll-like receptor signaling pathway intracellular signal transduction Fc-epsilon receptor signaling pathway regulation of I-kappaB kinase/NF-kappaB signaling regulation of mast cell degranulation protein autophosphorylation phosphatidylinositol-mediated signaling focal adhesion assembly T cell receptor signaling pathway positive regulation of release of sequestered calcium ion into cytosol cellular response to epidermal growth factor stimulus positive regulation of establishment of protein localization to plasma membrane extrinsic apoptotic signaling pathway glucose metabolic process cell surface receptor signaling pathway cell proliferation intrinsic apoptotic signaling pathway in response to oxidative stress regulation of acetyl-CoA biosynthetic process from pyruvate regulation of glucose metabolic process hypoxia-inducible factor-1alpha signaling pathway carbohydrate metabolic process phosphorylation mitophagy in response to mitochondrial depolarization
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