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PDK4 Polyclonal Antibody

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PDK4 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 4% PFA) fixed HepG2 cells using 12949-1-AP (PDK4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PDK4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 12949-1-AP (PDK4 Antibody) at dilution of 1:200 (under 40x lens).

PDK4 Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12949-1-AP (PDK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Product Details

12949-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:3,000	View 8 publications 8 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:500	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PDK4 Fusion Protein Ag3629 (61-412 aa encoded by BC040239) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.19 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: LPVRLANIL KEIDILPTQL VNTSSVQLVK SWYIQSLMDL VEFHEKSPDD QKALSDFVDT LIKVRNRHHN VVPTMAQGII EYKDACTVDP VTNQNLQYFL DRFYMNRIST RMLMNQHILI FSDSQTGNPS HIGSIDPNCD VVAVVQDAFE CSRMLCDQYY LSSPELKLTQ VNGKFPDQPI HIVYVPSHLH HMLFELFKNA MRATVEHQEN QPSLTPIEVI VVLGKEDLTI KISDRGGGVP LRIIDRLFSY TYSTAPTPVM DNSRNAPLAG FGYGLPISRL YAKYFQGDLN LYSLSGYGTD AIIYLKALSS ESIEKLPVFN KSAFKHYQMS SEADDWCIPS REPKNLAKEV AM (61-412 aa encoded by BC040239)

Target Information

PDK4 is a mitochondrial protein with a histidine kinase domain that is a member of the PDK/BCKDK protein kinase family. This protein is located in the matrix of the mitrochondria and it's expression is regulated by glucocorticoids, retinoic acid and insulin. PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Overexpression of PDK4 is associated with cell proliferation, drug resistance and poor prognosis in ovarian cancer.

Cancer management and research

Wang J,Qian Y,Gao M

Thu Mar 31 00:00:00 EDT 2022

Effects of functional β-glucan on proliferation, differentiation, metabolism and its anti-fibrosis properties in muscle cells.

International journal of biological macromolecules

Li Y,Fan Y,Pan H,Qian H,Qi X,Wu G,Zhang H,Xu M,Rao Z,Wang L,Ying H

Mon Oct 01 00:00:00 EDT 2018

AMPK/GSK3β/β-catenin cascade-triggered overexpression of CEMIP promotes migration and invasion in anoikis-resistant prostate cancer cells by enhancing metabolic reprogramming.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zhang P,Song Y,Sun Y,Li X,Chen L,Yang L,Xing Y

Sun Jul 01 00:00:00 EDT 2018

Down-regulation of PDK4 is Critical for the Switch of Carbohydrate Catabolism during Syncytialization of Human Placental Trophoblasts.

Scientific reports

Liu X,Zuo R,Bao Y,Qu X,Sun K,Ying H

Wed Aug 16 00:00:00 EDT 2017

Down-regulation of PDK4 is Critical for the Switch of Carbohydrate Catabolism during Syncytialization of Human Placental Trophoblasts.

Scientific reports

Liu X,Zuo R,Bao Y,Qu X,Sun K,Ying H

Wed Aug 16 00:00:00 EDT 2017

MiR-155 Enhances Insulin Sensitivity by Coordinated Regulation of Multiple Genes in Mice.

PLoS genetics

Lin X,Qin Y,Jia J,Lin T,Lin X,Chen L,Zeng H,Han Y,Wu L,Huang S,Wang M,Huang S,Xie R,Liang L,Liu Y,Liu R,Zhang T,Li J,Wang S,Sun P,Huang W,Yao K,Xu K,Du T,Xiao D

Sat Oct 01 00:00:00 EDT 2016

Enhancement of Glucose Metabolism via PGC-1α Participates in the Cardioprotection of Chronic Intermittent Hypobaric Hypoxia.

Frontiers in physiology

Li X,Liu Y,Ma H,Guan Y,Cao Y,Tian Y,Zhang Y

Mon Jul 04 00:00:00 EDT 2016

Effects of neonatal dexamethasone administration on cardiac recovery ability under ischemia-reperfusion in 24-wk-old rats.

Pediatric research

Jiang X,Ma H,Li C,Cao Y,Wang Y,Zhang Y,Liu Y

Fri Jul 01 00:00:00 EDT 2016

AMP-Activated Protein Kinase Interacts with the Peroxisome Proliferator-Activated Receptor Delta to Induce Genes Affecting Fatty Acid Oxidation in Human Macrophages.

PloS one

Kemmerer M,Finkernagel F,Cavalcante MF,Abdalla DS,Müller R,Brüne B,Namgaladze D

Mon Mar 21 00:00:00 EDT 2016

PPARγ isoforms differentially regulate metabolic networks to mediate mouse prostatic epithelial differentiation.

Cell death & disease

Strand DW,Jiang M,Murphy TA,Yi Y,Konvinse KC,Franco OE,Wang Y,Young JD,Hayward SW

Thu Aug 09 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial; kinase isozyme 4; lipoamide; pyruvate dehydrogenase; pyruvate dehydrogenase kinase 4; Pyruvate dehydrogenase kinase isoform 4; pyruvate dehydrogenase kinase, isoenzyme 4; pyruvate dehydrogenase kinase, isozyme 4; pyruvate dehydrogenase, lipoamide, kinase isozyme 4, mitochondrial; pyruvate dehydrogenate kinase 4

Gene Aliases: AV005916; PDHK4; PDK4

UniProt ID: (Human) Q16654, (Rat) O54937, (Mouse) O70571

Entrez Gene ID: (Human) 5166, (Rat) 89813, (Mouse) 27273

Molecular Function: non-receptor serine/threonine protein kinase