Immunofluorescent analysis of HeLa cells using a PDK4 polyclonal antibody (Product # PA5-13778) at a dilution of 1:10-50. Primary antibody was detected with goat anti-rabbit lgG, fluor-conjugated secondary antibody (green). Actin filaments have been labeled with red dye conjugated phalloidin.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 83-111 amino acids from the central region of human PDK4|
|Purification||Size-exclusion - Dialysis, Ammonium sulfate precipitation|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial; Pyruvate dehydrogenase kinase isozyme 4, mitochondrial; pyruvate dehydrogenase kinase, isoenzyme 4; pyruvate dehydrogenase, lipoamide, kinase isozyme 4, mitochondrial