|Western Blot (WB)||2 µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide made to an internal portion of the human protein (within residues 200-250).|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 30% glycerol|
|Contains||0.1% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
The target sequence has 94% sequence homology with rat and 88% sequence homology with mouse.
Suggested positive control: antigen standard for PDK4 (transient overexpression lysate), human heart protein lysate.
PDK4 is a mitochondrial protein with a histidine kinase domain that is a member of the PDK/BCKDK protein kinase family. This protein is located in the matrix of the mitrochondria and it's expression is regulated by glucocorticoids, retinoic acid and insulin. PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Protein Aliases: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial; isoenzyme 4; kinase isozyme 4; lipoamide; mitochondrial; pyruvate dehydrogenase; pyruvate dehydrogenase kinase; Pyruvate dehydrogenase kinase isoform 4; pyruvate dehydrogenase kinase, isoenzyme 4; pyruvate dehydrogenase, lipoamide, kinase isozyme 4, mitochondrial
Gene Aliases: PDHK4; PDK4
UniProt ID: (Human) Q16654
Entrez Gene ID: (Human) 5166