Immunofluorescent analysis of PDLIM4 showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a PDLIM4 polyclonal antibody (Product # PA5-28425) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 267 and 330 of Human PDLIM4|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-28425 targets PDLIM4 in WB applications and shows reactivity with Human samples.
The PA5-28425 immunogen is synthetic peptide corresponding to a region within amino acids 267 and 330 of Human PDLIM4.
This gene encodes a protein which may be involved in bone development. Mutations in this gene are associated with susceptibility to osteoporosis.
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