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Product Details
PA5-115733
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PDPK1(Accession O15530), corresponding to amino acid residues L350-S400.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2900368
Product Specific Information
Antibody detects endogenous levels of total PDPK1.
Target Information
PDPK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDPK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDPK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDPK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3-phosphoinositide dependent protein kinase-1; 3-phosphoinositide-dependent protein kinase 1; 3-phosphoinositide-dependent protein kinase 2 pseudogene; HGNC:8809; hPDK1; isoenzyme 1; kinase isoenzyme 1; lipoamide; mPDK1; OTTHUMP00000174525; OTTHUMP00000205076; PkB kinase; PkB kinase like gene 1; PkB-like 1; Protein kinase B kinase
Gene Aliases: PDK1; PDPK1; PDPK2; PDPK2P; PRO0461
UniProt ID: (Human) O15530, (Mouse) Q9Z2A0, (Rat) O55173
Entrez Gene ID: (Human) 5170, (Mouse) 18607, (Rat) 81745
protein serine/threonine kinase activity
3-phosphoinositide-dependent protein kinase activity
insulin receptor binding
protein binding
ATP binding
phospholipase activator activity
kinase activity
protein kinase binding
phospholipase binding
nucleotide binding
protein kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
stimulatory C-type lectin receptor signaling pathway
type B pancreatic cell development
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
negative regulation of protein kinase activity
hyperosmotic response
epidermal growth factor receptor signaling pathway
positive regulation of phospholipase activity
regulation of endothelial cell migration
negative regulation of cardiac muscle cell apoptotic process
cell migration
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
calcium-mediated signaling
actin cytoskeleton organization
platelet activation
negative regulation of transforming growth factor beta receptor signaling pathway
T cell costimulation
activation of protein kinase B activity
cellular response to insulin stimulus
negative regulation of toll-like receptor signaling pathway
intracellular signal transduction
Fc-epsilon receptor signaling pathway
regulation of I-kappaB kinase/NF-kappaB signaling
regulation of mast cell degranulation
protein autophosphorylation
phosphatidylinositol-mediated signaling
focal adhesion assembly
T cell receptor signaling pathway
positive regulation of release of sequestered calcium ion into cytosol
cellular response to epidermal growth factor stimulus
positive regulation of establishment of protein localization to plasma membrane
extrinsic apoptotic signaling pathway
signal transduction
phosphorylation
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